0.1% Gelatin Solution (ATCC® PCS-999-027)

Biosafety Level N/A
Applications
0.1% Gelatin Solution is used to coat cell culture flasks.
Product Format 100 ml
Storage Conditions 2 to 8°C
Comments
The use of coated flasks improves cell attachment for certain types of primary cells as well as certain continuous cell lines. This sterile-filtered solution contain 0.1% porcine gelatin in water.
Complete Growth Medium Preparing Gelatin-Coated Tissue Culture Flasks

For optimal cell attachment, cell culture flasks should be prepared before thawing the cells.

  1. Decontaminate the external surface of the 0.1% Gelatin Solution bottle by spraying with 70% ethanol.
  2. Using aseptic technique and working in a laminar flow hood or biosafety cabinet, add 1.0 mL of 0.1% Gelatin Solution per 10 cm2 of culture surface area (e.g., 2.5 mL if using a T-25 flask). 
  3. Rock culture flask to coat surface; place in a 37°C incubator (with or without 5% CO2) for at least 30 minutes, and up to overnight.
  4. Aspirate the excess gelatin solution from the culture flasks using sterile technique.
  5. Add 5.0 mL of complete growth medium per 25 cm2 of culture surface area (e.g., 5.0 mL if using a T-25 flask).
  6. Place the gelatin-coated flasks in a 37°C, 5% CO2 incubator for at least one hour to equilibrate before inoculating with the cell suspension.   
Volume 100 ml
Sterility Tests
Negative for bacteria, fungi and yeast
Functional Tests Each lot is assessed for acceptable attachment of human primary cells.
A Certificate of Analysis (COA) is available upon request for each lot of 0.1% Gelatin Solution.
C of A
Certificate of Analysis
Certificate of Analysis