CellMatrix Basement Membrane Gel (ATCC® ACS-3035)

Product Format frozen 5 mL
Storage Conditions -80°C or colder
Comments
Each lot of CellMatrix Gel is shown to promote the attachment of hESCs/hiPSCs and to maintain hESCs/hiPSCs in a pluripotent state as determined by the expression of Oct-4 and Nanog pluripotent markers.
Store at -80°C in aliquots. Avoid repeated freezing and thawing.
Biosafety Level 1
Quality Control Specifications Functional testing: Each lot of CellMatrix Gel is shown to promote the attachment of hESCs/hiPSCs and to maintain hESCs/hiPSCs in a pluripotent state as determined by the expression of Oct-4 and Nanog pluripotent markers.  

Sterility testing: No bacterial or fungal growth is detected. Negative for the presence of mycoplasma and 31 pathogens and viruses, including LDEV, as demonstrated by PCR. Endotoxin concentrations are less than 8 EU/mL by LAL assay. 

Concentration: 12 to 18 mg/mL. Please refer to the certificate of analysis for the lot-specific concentration. 

Storage Buffer: Product is stored in Dulbecco’s Modified Eagle’s Medium (DMEM) with 10 µg/mL gentamycin, without phenol red. A Certificate of Analysis is available upon request.

Sterility Testing
No bacterial or fungal growth is detected. Negative for the presence of mycoplasma and 31 pathogens and viruses, including LDEV, as demonstrated by PCR. Endotoxin concentrations are less than 8 EU/mL by LAL assay.