Cell Supplements

  • Mesenchymal Stem Cell Growth Kit for Bone Marrow-derived MSCs (ATCC® PCS-500-041)

    ATCC® Number: PCS-500-041
    Quantity: 1 kit
    Storage: -20°C (or -70°C for long-term storage)

    For-Profit:   $118.00 Non-Profit:   $118.00

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    Product Format: frozen 1 kit
    Comments:

    The Mesenchymal Stem Cell Growth Kit for Bone Marrow-derived MSCs contains components that when added to Mesenchymal Stem Cell Basal Medium for Adipose, Umbilical and Bone Marrow-derived MSCs (ATCC PCS-500-030) create a complete cell culture environment for mesenchymal stem cells isolated from normal human bone marrow. The final concentration for each component in complete mesenchymal stem cell growth medium is as follows:

    • rh FGF basic: 125 pg/mL
    • rh IGF-1: 15 ng/mL 
    • Fetal Bovine Serum: 7% 
    • L-Alanyl-L-Glutamine: 2.4 mM   
  • L-Glutamine Solution, 200 mM (ATCC® 30-2214)

    ATCC® Number: 30-2214
    Quantity: 100 mL
    Storage: Store L-glutamine solution frozen at -20°C or colder. Avoid repeated freeze-thaws by dispensing and storing in aliquots.
    Applications: L-Glutamine is an essential amino acid required by virtually all mammalian and insect cells grown in culture.

    L-Glutamine is also more labile in cell culture solutions than other amino acids. The rate and extent of L-glutamine degradation are related to storage temperatures, age of the product, and pH.

    All cell culture media are formulated to contain appropriate amounts of L-glutamine to support cell growth well beyond the expiration date of the media. However, if L-glutamine is suspected to be a limiting factor during cell culture, a simple test of 'spiking' the medium with a small amount of L-glutamine will determine whether or not more is required. Simply add a small amount of L-glutamine (~ 2 mM final concentration) to the culture medium. If the cell growth rate increases, L-glutamine is most likely deficient and more should be added.

    For-Profit:   $18.00 Non-Profit:   $18.00

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    Product Format: frozen 100 mL
    Comments:

    Added as a supplement to cell culture media. L-Glutamine is an essential amino acid required by virtually all mammalian and insect cells grown in culture. L-Glutamine is also more labile in cell culture solutions than other amino acids. The rate and extent of L-glutamine degradation are related to storage temperatures, age of the product, and pH. 

    Note: L-glutamine degradation results in the build-up of ammonia which can have a deleterious effect on some cell lines. Use caution when adding more L-glutamine than called for in the original medium formulation (2 to 4 mM is standard). For most cell lines, ammonia toxicity is more critical for cell viability than L-glutamine limitation.

    For more information, please contact ATCC Technical Service.

  • MEM Nonessential Amino Acid Solution, 100x Solution (ATCC® 30-2116)

    ATCC® Number: 30-2116
    Quantity: 100 mL
    Storage: Store MEM Nonessential Amino Acid Solution at 2°C to 8°C
    Applications:

    Added as a supplement to minimal basal media. The nonessential amino acids in this solution are 100 times the concentration found in MEM-alpha Medium.

    For-Profit:   $14.00 Non-Profit:   $14.00

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    Formulation: Formulation
    Product Format: 100 mL
    Comments:
    Added as a supplement to minimal basal media. Contains the nonessential amino acids found in MEM-alpha medium at 100x concentration.
  • L-Alanyl-L-Glutamine, 200 mM Solution in 0.85% NaCl. (ATCC® 30-2115)

    ATCC® Number: 30-2115
    Quantity: 100 mL
    Storage: Store L-alanyl-L-glutamine solution at 2°C to 30°C.
    Applications:

    L-glutamine (200 mM solution in 0.85% NaCl) is an essential amino acid required by virtually all mammalian and insect cells grown in culture. L‑alanyl-L-glutamine can be used as a direct substitute for L-glutamine at equimolar concentrations (2 to 4 mM for most applications). Whereas L-glutamine is labile in cell culture medium and its degradation results in a deleterious build-up of ammonia, the L-alanyl-L-glutamine dipeptide is very stable with minimal ammonia production. Once inside the cell, the dipeptide is split by aminopeptidases into L-alanine and L-glutamine.

    For-Profit:   $27.00 Non-Profit:   $27.00

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