Transfection Reagents

  • GeneXPlus Transfection Reagent (ATCC® ACS-4004)

    ATCC® Number: ACS-4004
    Quantity: 1 mL
    Storage: -20°C
    Applications:

    Transient transfection, stable transfection, gene expression, protein production, genetic manipulation.  Suitable for transfection of Bone Marrow MSCs (PCS-500-012), Dermal Microvascular Endothelial cells (PCS-110-010), THP-1 cells (TIB-202), Raw 264.7 cells (TIB-71), and SH-SY5Y cells (CRL-2266).

    For-Profit:   $350.00 Non-Profit:   $350.00

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    Product Format: 1 mL
    Comments:

    This reagent affords high levels of gene expression in a variety of cell types and is suitable for both transient and stable transfection. GeneXPlus works effectively in large scale reactions, producing high protein yields. Since the reagent is composed of animal-origin free components and is serum compatible, there is no need for any culture medium change after transfection.

     

    GeneXPlus Transfection Reagent has been optimized for use with HEK293T/17 SF suspension cells (ATCC ACS-4500) and HEKPlus SFM (ATCC ACS-4004) to reproducibly express a wide range of proteins at high levels.

  • HEKPlus Boost Solution (ATCC® ACS-4003)

    ATCC® Number: ACS-4003
    Quantity: 50 mL
    Storage: Store at 2°C to 8°C. Do not freeze. Protect from light.

    For-Profit:   $35.00 Non-Profit:   $35.00

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    Product Format: 50 mL
    Comments: The addition of peptone following transfection has been shown to increase extracellular protein expression yields in transient transfection protocols.

    HEKPlus Boost Solution should be added post transfection to a final concentration of 10% in complete HEKPlus SFM. The solution contains 0.1% Poloxamer 188, a surfactant shown to enhance cell yield and reduce cell aggregation. HEKPlus Boost Solution also contains human transferrin and recombinant human insulin.

    This solution has been optimized for use with ATCC® HEK293T/17 SF suspension cells (ATCC ACS-4500), HEKPlus SFM medium (ATCC ACS-4002) and GeneXPlus transfection reagent (ATCC ACS-4004) to reproducibly express a wide range of proteins at high levels.

  • TransfeX™ Transfection Reagent (ATCC® ACS-4005)

    ATCC® Number: ACS-4005
    Quantity: 500 μL
    Storage: Store at 2°C to 8°C
    Applications:

    Transient transfection, stable transfection, gene expression, protein production, genetic manipulation.

    For-Profit:   $225.00 Non-Profit:   $225.00

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    Product Format: 500 μL
    Comments:

    Reagent is designed for transfection of primary cells and hard-to-transfect cell lines.  TransfeX Transfection Reagent provides high efficiency with low cell mortality.

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