TransfeX™ Transfection Reagent (ATCC® ACS-4005)

Permits and Restrictions

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Product Format 500 μL
Storage Conditions Store at 2°C to 8°C
Shipping Information Refrigerated Bricks
Applications

Transient transfection, stable transfection, gene expression, protein production, genetic manipulation.

Comments

Reagent is designed for transfection of primary cells and hard-to-transfect cell lines.  TransfeX Transfection Reagent provides high efficiency with low cell mortality.

Biosafety Level N/A
Quality Control Specifications The molecular weight was determined by Mass Spectroscopy to be consistent with the expected structure.
Sterility Testing No evidence  of bacterial or fungal contamination. Mycoplasma testing was negative for the presence of mycoplasma.
Functional Testing HeLa cells are transfected with a lacZ reporter plasmid. The peak activity and the cytotoxicity for the test lot is determined in a beta-galactosidase functional assay and compared to control lot.
Basic Documentation
Other Documentation
Restrictions

This product's use is governed by a Limited Use Label License. For information on obtaining a license to use this product for purposes other than those permitted in the Limited Use Label License, please contact the ATCC Licensing Group at licensing@atcc.org.

FAQs
  1. Variable Transfection Reaction Size
    • Yes, TransfeX is suitable for scaling up transfection volumes as long as  the same ratio of DNA of TransfeX reagent is maintained. 

    Date Updated: 3/27/2014
  2. Optimize transfection conditions
    • Follow the recommended transfection condition for each cell type.
    • Monitor cellular toxicity at days 1 and 2 compared to untransfected cells.
    • Reduce the a...

    Date Updated: 3/27/2014
  3. Transfection media concerns
    • TransfeX  has been optimized for intracellular delivery of nuclei acids into cultured...

    Date Updated: 3/27/2014
  4. Toxicity and transfection
    • We usually see more cellular toxicity at day 2 than at day 1 post-transfection.  Reducing the amount of DNA and transfection reagent added to the cells and increasing cell dens...

    Date Updated: 3/27/2014
  5. Lower than expected transfection efficiency
    • Verify that cells are contaminant-free.  Mycoplasma contamination can cause a suppression of transfection efficiency.
    • <...>

    Date Updated: 3/27/2014
  6. Cell-specific transfection toxicity
    • 3T3-L1 and MDA-MB-231 cells are not as resilient to the reagent and may exhibit cytotoxic effects.  Refer to the important guidelines on the cell line specific product sheet wh...

    Date Updated: 3/27/2014
  7. Different vectors effect transfection efficiency
    • Different promoters work better with certain cell types.  CMV, though a strong promoter, may not be optimal for all cell types.  For example, EF-1α is a better promo...

    Date Updated: 3/27/2014