Geobacillus sp. (ATCC® 15952)

Strain Designations: NCA 5520  /  Type Strain: no  /  Biosafety Level: 1

Deposited As Bacillus stearothermophilus Donk
Strain Designations NCA 5520
Application
Degrades penicillin G benzylpenicillin
Produces muramoylpentapeptide carboxypeptidase D-alanine carboxypeptidase
Biotechnology
Type Strain no
Biosafety Level 1
Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol® no
Type Strain no
Medium ATCC® Medium 3: Nutrient agar or nutrient broth
Growth Conditions
Temperature: 50°C
Atmosphere: Aerobic
Name of Depositor Nat. Canners Assn.
References

Hammarstrom S, Strominger JL. Degradation of penicillin G to phenylacetylglycine by D-alanine carboxypeptidase from Bacillus stearothermophilus. Proc. Natl. Acad. Sci. USA 72: 3463-3467, 1975. PubMed: 1059132

Despreaux CW, Manning RF. The dacA gene of Bacillus stearothermophilus coding for D-alanine carboxypeptidase: cloning, structure and expression in Escherichia coli and Pichia pastoris. Gene 131: 35-41, 1993. PubMed: 8370539

Cross References

Nucleotide (GenBank) : X68587 B.stearothermophilus dacA gene for D-alanine carboxypeptidase.