L-Alanyl-L-Glutamine, 200 mM Solution in 0.85% NaCl. (ATCC® 30-2115)

Product Format 100 mL
Storage Conditions Store L-alanyl-L-glutamine solution at 2°C to 30°C. (can be stored refrigerated or at ambient temperature)
Applications

L-glutamine (200 mM solution in 0.85% NaCl) is an essential amino acid required by virtually all mammalian and insect cells grown in culture. L‑alanyl-L-glutamine can be used as a direct substitute for L-glutamine at equimolar concentrations (2 to 4 mM for most applications). Whereas L-glutamine is labile in cell culture medium and its degradation results in a deleterious build-up of ammonia, the L-alanyl-L-glutamine dipeptide is very stable with minimal ammonia production. Once inside the cell, the dipeptide is split by aminopeptidases into L-alanine and L-glutamine.

Biosafety Level 1
Quality Control Specifications Lot specific results are available in the Certificate of Analysis, which is available at www.atcc.org or by contacting ATCC technical service.

SPECIFICATION CERTIFICATE

Test

Specification

Sterility Testing1

Pass

Osmolality

400 to 500 mOsm

pH

5.0 -6.0

Mycoplasma2

Negative

Endotoxin3

For information

Cell Growth Testing4

Pass

  1. Tested by membrane filtration techniques in accordance with United States Pharmacopeia Chapters [71] and [1211].
  2. Large-volume method of Kern and Barile as per “Isolation of mycoplasmas from cell culture by agar and broth techniques,” Barile MF and McGarrity GJ. In: Tully JG and Razin S, eds. Methods in Mycoplasmology, Vol. II. New York: Academic Press; 1983; pp. 159-165.
  3. Chromogenic LAL assay using guidelines outlined by the manufacturer and FDA (December 1987), “Validation of the limulus amebocyte lysate test as an end product endotoxin test for human and animal parenteral drugs, biological products, and medical devices.”
  4. Growth-promotion capability is analyzed by measuring the fold increase over time of the appropriate cell lines according to predetermined standards (harvest to plant ratio) for rate of growth, density, atypical morphology, and evidence of cytotoxicity.