Trypanosoma rangeli Tejera (ATCC® 30033)

Organism: Trypanosoma rangeli Tejera  /  Depositor: EJ Tobie

Strain Designations E1 Tocuyo, substrain JJ8/8
Application
Vector borne research
Biosafety Level 2
Isolation
substrain of T. rangeli ATCC 30032 (1968)
Product Format frozen
Type Strain no
Medium Medium 1012: Diphasic blood agar medium
Growth Conditions
Temperature: 25.0°C
Duration: axenic
Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
Subcultivation
Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
Name of Depositor EJ Tobie
Year of Origin 1968