pRK2013 (ATCC® 37159)

Applications: mobilization helper plasmid  /  Depositors: EW Nester

Designations pRK2013
Depositors EW Nester
Biosafety Level 1
Vector Information
Size (kb): 48.0
Vector: pRK2013 (plasmid)
Construction: pPRK212.2, pDF1
Marker(s): kanR
Construct size (kb): 48.0
Features:
marker(s): kanR
replicon: RK2
Applications
mobilization helper plasmid
Comments
This is a helper plasmid for mobilization of non-self-transmissible plasmids.
Media Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin
Growth Conditions
Temperature: 37°C
References

Knauf VC, Nester EW. Wide host range cloning vectors: a cosmid clone bank of an Agrobacterium ti plasmid. Plasmid 8: 45-54, 1982. PubMed: 6292969

Figurski DH, Helinski DR. Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in trans. Proc. Natl. Acad. Sci. USA 76: 1648-1652, 1979. PubMed: 377280

Shipped frozen
Product Sheet
Product Sheet
Product Sheet