Size (kb): 9.0719995498657230
Vector: pMMB207 (plasmid)
Promoters: Promoter tac
Construction: pMMB206, pMMB67EH
Construct size (kb): 9.071999549865723
Features: marker(s): cmlR
replicon: oriV, oriT (IncQ)
transcription terminator: rrnB
Restriction digests of the clone give the following sizes (kb): EcoRI--9.1; EcoRV--5.3, 3.9; SspI--4.1, 1.8, 1.6, 1.0, 0.7; EcoRI/MluI--8.3, 0.8.
Does not have a start codon at a suitable distance after the ribosome-binding site, so translation will be efficient only if the insert contains an RBS followed appropriately by a start codon.
pMMB207 (ATCC 37809)
and pMMB208 (ATCC 37810)
differ in the orientation of the multiple cloning site with pMMB207 having the EcoRI site near the promoter and pMMB208 having the HindIII site nearer.
Constructed by replacing the MluI/XmnI fragment of pMMB206 (taclacUV5 promoter, M13mp8 polylinker, lacZalpha) with the MluI/XmnI fragment of pMMB67EH (tac promoter, M13mp18 polylinker without lacZalpha).
Morales VM, et al. A series of wide-host-range low-copy-number vectors that allow direct screening for recombinants. Gene 97: 39-47, 1991. PubMed: 1847347
Shipped: Freeze dried E. coli containing the plasmid.