p426 GAL1 plasmid in E. coli (ATCC® 87333)

Applications: YE-type (episomal) shuttle vectorYX-type (expression) shuttle vector  /  Depositors: M Funk

Designations p426 GAL1 plasmid in E. coli
Depositors M Funk
Biosafety Level 1
Vector Information
Size (kb): 6.417
Vector: p426 GAL1 (plasmid)
Promoters: Promoter for expression GAL1
Construction: pRS426 (ATCC 77107), GAL1 promoter
Marker(s):URA3,ampR
Construct size (kb): 6.417
Features: marker(s): URA3
marker(s): ampR
promoter for expression: GAL1
replicon: 2 micron
MCS: XhoI...XpeI
terminator: CYC1
Applications
YE-type (episomal) shuttle vector
YX-type (expression) shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): SacI/XbaI--3.2, 2.8, 0.5; EcoRI--6.4; KpnI--6.4.
Expression from the galactokinase (GAL1) promoter is tightly repressed by glucose and is strongly induced by galactose.
High copy number shuttle expression vector.
One of 32 yeast expression vectors (ATCC 87318-87349) differing in promoter, selection marker and replicon.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Mumberg D, et al. Regulatable promoters of Saccharomyces cerevisiae: comparison of transcriptional activity and their use for heterologous expression. Nucleic Acids Res. 22: 5767-5768, 1994. PubMed: 7838736

Related Products
component of:ATCC 87670
Shipped frozen
Shipping Information

 Frozen glycerol stock of  E. coli containing the plasmid