Synthetic Treponema pallidum DNA (ATCC® BAA-2642SD)

Biosafety Level: 1

Application ATCC® Genuine Nucleics can be used for assay development, verification, validation, monitoring of day to day test variation, and lot to lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine bacterial load.
Biosafety Level 1
Product Format frozen
Specification range: 1 x 105 to 1 x 106 copies/µL
100 µL per vial with Biomatrica DNAstable
Storage Conditions -20°C or colder
Intended Use This product is intended for research use only. It is not intended for any animal or human therapeutic or diagnostic use. The synthetically engineered sequence of the product constitutes intellectual property belonging to ATCC. Unauthorized use, including sequencing, modification, or reverse-engineering, of the product is expressly prohibited without prior ATCC consent.

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Preceptrol® no
Comments Preparation includes fragments from the polA, tpr, 23S gene, arp, 16S gene, flaA, 47kDa protein gene, and bmp.

The following primers and probe can be used with this preparation RefKoek AG, et al. Specific and sensitive diagnosis of syphilis using a real-time PCR for Treponema pallidum. Clin. Microbiol. Infect. 12(12): 1233-1236, 2006. PubMed: 17121633:
Forward primer: polA-116777-F
Reverse primer: polA-116854-R
Probe: polA-116825-P
Special Collection DNA
References

Koek AG, et al. Specific and sensitive diagnosis of syphilis using a real-time PCR for Treponema pallidum. Clin. Microbiol. Infect. 12(12): 1233-1236, 2006. PubMed: 17121633