Escherichia coli (Migula) Castellani and Chalmers (ATCC® BAA-525)

Strain Designations: ET12567  /  Type Strain: no  /  Biosafety Level: 1

Permits And Restrictions

View Permits

Classification Enterobacteriaceae, Escherichia
Strain Designations ET12567
Application
It is the key host strain for streptomyces workers worldwide.

This is a host for isolation of non-methylated DNA for transfer to other species.

This strain is used as a host to isolate DNA and plasmids for transformation into Streptomyces or any other organism that has a methylation-sensitive restriction system.

Isolation
Derived from existing strain
Type Strain no
Biosafety Level 1
Product Format freeze-dried
Preceptrol® no
Genotype F- dam-13::Tn9 dcm-6 hsdM hsdR zjj-202::Tn10 recF143 galK2 galT22 ara-14 lacY1 xyl-5 leuB6 thi-1 tonA31 rpsL136 hisG4 tsx-78 mtl-1 glnV44 (Douglas J MacNeil, personal communication)
Type Strain no
Colony Morphology Colonies on #1675 agar are circular with irregular edges, glistening, and opaque.
Medium ATCC® Medium 1675: LB medium (ATCC medium 1065) with 10 mcg/ml Chloramphenicol
Growth Conditions
Temperature: 37°C
Atmosphere: Aerobic
Genotype F- dam-13::Tn9 dcm-6 hsdM hsdR zjj-202::Tn10 recF143 galK2 galT22 ara-14 lacY1 xyl-5 leuB6 thi-1 tonA31 rpsL136 hisG4 tsx-78 mtl-1 glnV44 (Douglas J MacNeil, personal communication)
Colony Morphology Colonies on #1675 agar are circular with irregular edges, glistening, and opaque.
Name of Depositor DJ MacNeil, T MacNeil
Special Collection NSF - Bacteriology
Isolation
Derived from existing strain
References

MacNeil DJ, et al. Analysis of Streptomyces avermitilis genes required for avermectin biosynthesis utilizing a novel integration vector. Gene 111: 61-68, 1992. PubMed: 1547955

constructed from strain GM2929 by T. MacNeil

Douglas J MacNeil, personal communication

Douglas J MacNeil, personal communication

Douglas J MacNeil, personal communication

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation