A7 [M2A7] (ATCC® CRL-2500)

Organism: Homo sapiens, human  /  Cell Type: Melanoma  / 

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Organism Homo sapiens, human
Cell Type Melanoma
Product Format frozen
Morphology melanocytic
Culture Properties adherent
Biosafety Level 1
Applications
M2 cells are severely deficient in filamin-1 mRNA and protein.
The filament-1 gene on the X chromosome is present in these cells.
A7 cells are useful as a control for studying these systems.
Storage Conditions liquid nitrogen vapor phase
Genes Expressed
filamin-1 (actin-binding protein ABP or ABP-280)
Cellular Products
filamin-1 (actin-binding protein ABP or ABP-280)
Comments
M2 cells are severely deficient in filamin-1 mRNA and protein. Filamin-1, also known as actin-binding protein (ABP, APB-280), cross-links actin filaments in vitro and connects these filaments to plasma membrane glycoproteins.
The filament-1 gene on the X chromosome is present in these cells.
ABP (filamin-1) was transfected into M2 cells in a mammalian expression vector (LK444) containing full length human ABP recombinant DNA (LK-APB).
A7 cells express levels of filamin-1 comparable to wild-type filamin-1 containing melanoma cells.
Filamin-1 is essential for cell migration and has been shown to bind partners in cell signal transduction, cell membrane sorting and cytoskeleton-membrane association.
A7 cells are useful as a control for studying these systems.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium:
  • 10 mM HEPES
  • 0.5 mg/ml G418
  • fetal bovine serum to a final concentration of 10%

Subculturing
Protocol: Remove medium, and rinse with 0.25% trypsin - 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: culture medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
Name of Depositor TP Stossel
References

Cunningham CC, et al. Actin-binding protein requirement for cortical stability and efficient locomotion. Science 255: 325-327, 1992. PubMed: 1549777

Cunningham CC. Actin polymerization and intracellular solvent flow in cell surface blebbing. J. Cell Biol. 129: 1589-1599, 1995. PubMed: 7790356

Cantiello HF, et al. Actin-binding protein contributes to cell volume regulatory ion channel activation in melanoma cells. J. Biol. Chem. 268: 4596-4599, 1993. PubMed: 8383118

Byers HR, et al. Cell migration and actin organization in cultured human primary, recurrent cutaneous and metastatic melanoma. Time-lapse and image analysis. Am. J. Pathol. 139: 423-435, 1991. PubMed: 1867326

A7 is a stably transfected cell line derived from the M2 (RPM-MC) cell line. M2 was established from the tumor of a patient with malignant melanoma.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Cunningham CC, et al. Actin-binding protein requirement for cortical stability and efficient locomotion. Science 255: 325-327, 1992. PubMed: 1549777

Cunningham CC. Actin polymerization and intracellular solvent flow in cell surface blebbing. J. Cell Biol. 129: 1589-1599, 1995. PubMed: 7790356

Cantiello HF, et al. Actin-binding protein contributes to cell volume regulatory ion channel activation in melanoma cells. J. Biol. Chem. 268: 4596-4599, 1993. PubMed: 8383118

Byers HR, et al. Cell migration and actin organization in cultured human primary, recurrent cutaneous and metastatic melanoma. Time-lapse and image analysis. Am. J. Pathol. 139: 423-435, 1991. PubMed: 1867326

A7 is a stably transfected cell line derived from the M2 (RPM-MC) cell line. M2 was established from the tumor of a patient with malignant melanoma.