A375-MA1 (ATCC® CRL-3222)

Organism: Homo sapiens, human  /  Tissue: skin  /  Disease: malignant melanoma

Permits and Restrictions

View Permits

Organism Homo sapiens, human
Tissue skin
Product Format frozen 1.0 mL
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease malignant melanoma
Age 54 years
Gender female
Applications This cell line is useful to study the mechanisms of metastasis. It has been used with microarray analyses to identify metastasis-specific genes using a functional genomics approach and in proteomics analyses.
Storage Conditions liquid nitrogen vapor phase
Derivation This increased metastatic cell line was derived using an in vivo selection process of highly metastatic cells from a population of poorly metastatic tumor cells, A375  (ATCC CRL-1619). The A375-M1 (ATCC CRL-3222) cell line was derived by i.v. injection of A375 cells into nude mice. Lung metastases were harvested and amplified in vitro as cell lines. The A375-M1 cell line was reinjected into mice for a second round of selection, lung metastases harvested and amplified in vitro as A375-M2 (ATCC CRL-3223) cells. The A375-M1 and A375-M2 cell lines were  transfected with a plasmid containing the ecotropic receptor for murine retrovirus and selected for neomycin resistance. This is useful for RNAse protection assays.
Clinical Data
54 years
female
Tumorigenic yes
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
1. Remove and discard culture medium.
2. Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) (ATCC 30-2200) or 0.05% (w/v) Trypsin-0.02% EDTA solution (ATCC PCS-999-003) to remove all traces of serum that contains trypsin inhibitor.
3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
5. Add appropriate aliquots of the cell suspension to new culture vessels.
6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation Freeze medium: Complete growth medium supplemented with 10% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Volume 1.0 mL
STR Profile Amelogenin: X
CSF1PO: 11,12
D13S317: 11,14
D16S539: 9
D5S818: 12
D7S820: 9
THO1: 8
TPOX: 8,10
vWA: 16,17
Name of Depositor R Hynes
Year of Origin 2004
Year of Deposit 2014
References

Clark EA, et al. Genomic analysis of metastasis reveals an essential role for RhoC. Nature 406: 532-535, 2000. PubMed: 10952316

Lei X, et al. Gene Expression Changes in an Animal Melanoma Model Correlate with Aggressiveness of Human Melanoma Metastases. Mol. Cancer Res. 6(5): 760-769, 2008. PubMed: 18505921

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Clark EA, et al. Genomic analysis of metastasis reveals an essential role for RhoC. Nature 406: 532-535, 2000. PubMed: 10952316

Lei X, et al. Gene Expression Changes in an Animal Melanoma Model Correlate with Aggressiveness of Human Melanoma Metastases. Mol. Cancer Res. 6(5): 760-769, 2008. PubMed: 18505921