TeloHAEC (ATCC® CRL-4052)

Organism: Homo sapiens, human  /  Cell Type: endothelial  /  Tissue: aorta  /  Disease: normal

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Organism Homo sapiens, human
Tissue aorta
Cell Type endothelial
Product Format frozen
Morphology endothelial
Culture Properties adherent
Biosafety Level 2  [Cells containing SV40 viral DNA sequences]
Disease normal
Age 23
Gender female
Applications TeloHAEC (ATCC CRL-4052) cells retain important endothelial cell characteristics such as  CD31/PECAM-1 marker expression and LDL functional uptake; the cells also show effective inflammatory response upon TNFα treatment and increase proliferation upon VEGF stimulation.  When co-cultured with fibroblasts, TeloHAEC cells can also form neoangiogenic tubular networks in vitro, which are responsive to VEGF stimulation and suramin inhibition.
Storage Conditions liquid nitrogen vapor phase
Karyotype This is a diploid cell line of female origin with a consistent normal kayrotype at low and high passages
Images CRL-4052 Cell Micrograph Angiogensis Co-Culture with BJ
Derivation TeloHAEC is a clonal cell line immortalized by stably expressing human telomerase catalytic subunit hTERT
Clinical Data female 

34

Antigen Expression Positive for CD31/PECAM-1 expression  and capable of uptaking Low Density Lipoprotein (LDL).
Complete Growth Medium The base medium for this cell line is Vascular Cell Basal Medium (ATCC PCS-100-030), supplemented with Vascular Endothelial Cell Growth Kit-VEGF (ATCC PCS-100-041).   Optional: Add 0.3ug/mL Puromycin(Santa Cruz Biotech: sc-108071A). Note: Do not filter complete medium.
Subculturing Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation solutions for culture vessels of other sizes.

Subculture when the culture is about 90% confluent.
  1. Remove and discard spent medium.
  2. Briefly rinse the cells with Dulbecco's Phosphate Buffered Saline (DPBS, ATCC 30-2200) and discard rinse solution.
  3. Add 2.0 to 3.0 mL room temperature Trypsin-EDTA for Primary Cells (ATCC PCS-999-003) to the flask. Incubate at 37°C for 5 min (until cells have detached).
  4. Neutralize trypsin by adding an equal volume of room temperature 2% FBS in DPBS.
  5. Centrifuge cells at 250 x g for 5 min at room temperature.
  6. Remove supernatant. Resuspend pellet in 6.0 to 8.0 mL Complete Growth Medium.
  7. Count cells, and seed 5.0 x 103 to 8.0 x 103 viable cells/cm2 to new culture vessels.
Medium Renewal: Every 2-3 days.
Cryopreservation 90% FBS, 10% DMSO
Culture Conditions Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
STR Profile

D5S818:  12        
D13S317: 9, 12
D7S820: 10, 11        
D16S539: 12, 13
vWA: 15, 16             
Amelogenin:  X
TPOX: 8
CSF1PO: 11, 12
TH01: 6, 8
Population Doubling Level (PDL) As part of our quality control, we have tested this cell line for its ability to grow for a minimum of 15 population doublings after recovery from cryopreservation. We have also compared its karyotype, telomerase expression level, growth rate, morphology and tissue-specific markers when first recovered from cryopreservation with that of cells at 10+ population doublings to ensure that there is no change in these parameters and that the cells are capable of extended proliferation.
Name of Depositor ATCC
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
  • License agreement required for commercial customer uses.
  • This material is distributed for research purposes only. A signed addendum to the ATCC Material Transfer Agreement must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
FAQ's
  1. Subculture TeloHAEC cells
    Subculture the TeloHAEC (ATCC® CRL-4052 ™) cells when they reach 80% to 90% confluence.
    Date Updated: 4/2/2014
  2. TeloHAEC culture medium
    Vascular Cell Basal Medium (ATCC® PCS-100-030 ™) supplemented with Vascular Endothelial Cell Growth Kit-VEGF (ATCC® PCS-100-041 ™).
    Date Updated: 4/2/2014
  3. Optimal seeding density for TeloHAEC cells
    The TeloHAEC cells (ATCC® CRL-4052 ™) seeded at 5 x 10
    Date Updated: 4/2/2014
  4. TeloHAEC cells retain characteristics


    Date Updated: 4/2/2014

  5. Antibiotics in TeloHAEC medium

    The culture medium for the TeloHAEC cells should be supplemented with puromycin to ensure expression of the hTERT.  Add puromycin to a final concentration of 0.3 µg/mL.


    Date Updated: 4/2/2014
  6. Better tube formation in tubulogenesis assay
    CellMatrix Basement Membrane Gel (ATCC® ACS-3035™) is recommended for the tubulogenesis assay. Major components include laminin, collagen IV, entactin and heparin sulfate proteoglycan. ...
    Date Updated: 4/2/2014
  7. Primary vs. immortalized vascular endothelial cells
    Primary vascular endothelial cells may grow for 15 population doublings (PD) before they become senescent and undergo growth arrest.  The hTERT-immortalized vascular endothelial cell lines a...
    Date Updated: 4/2/2014
Restrictions

This material is subject to claims under U.S. Patent Nos. 6,261,836 and 6,337,200, other pending patent applications, and foreign counterparts thereof. It is required that either the Addendum for Commercial and For-Profit Organizations or the Addendum for Noncommercial and Academic Organizations, as appropriate, be signed and returned to ATCC before shipment.