NCI-H1819 [H1819] (ATCC® CRL-5897)

Organism: Homo sapiens, human  /  Tissue: lung; derived from metastatic site: lymph node  /  Disease: adenocarcinoma

Permits and Restrictions

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Organism Homo sapiens, human
Tissue lung; derived from metastatic site: lymph node
Product Format frozen
Culture Properties suspension
Biosafety Level 1
Disease adenocarcinoma
Age 55 years
Gender female
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Derivation
The cell line was established from a patient who had received prior chemotherapy and radiation therapy. 
Clinical Data
55 years
Caucasian
female
The patient was a smoker (80 pack years).
Complete Growth Medium ACL-4 medium (serum-free)
The base medium for this cell line is ATCC formulated DMEM: F12 Medium Catalog No. 30-2006. To make the complete growth medium, add the following components to the base medium:
  • 0.02 mg/ml insulin
  • 0.01 mg/ml transferrin
  • 25 nM sodium selenite (final conc.)
  • 50 nM Hydrocortisone (final conc.)
  • 1 ng/ml Epidermal Growth Factor (do not filter)
  • 0.01 mM ethanolamine (final conc.)
  • 0.01 mM phosphorylethanolamine (final conc.)
  • 100 pM triiodothyronine (final conc.)
  • 0.5% (w/v) bovine serum albumin (final conc.)
  • 10 mM HEPES
  • 0.5 mM sodium pyruvate (final conc.)
  • extra 2mM L-glutamine (for final conc. of 4.5mM)

Subculturing

Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively cultures can be established by centrifugation with subsequent resuspension in fresh medium. Cultures grow as floating aggregates of round cell clusters with a few attached cells.

Note: CRL-5897 grows as aggregates/clusters in suspension.  Do not attempt to disassociate clusters as this will most likely result in decreased cell  viability.  Accurate cell counts are not possible or necessary.

Medium Renewal: Add medium as cell density increases
Cryopreservation
Freeze medium: Culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
STR Profile
Amelogenin:X
CSF1PO:12,14
D13S317:11
D16S539:10
D5S818:10,12
D7S820:9,10
THO1:9.3
TPOX:8
vWA: 16
Name of Depositor AF Gazdar, JD Minna
Year of Origin January, 1988
References

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

80 pack years

Basic Documentation
Restrictions

The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. A. Gazdar and Dr. J. Minna and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 699-8056, FAX (214) 688-7233.

References

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

80 pack years