Primary Bronchial Tracheal Smooth Muscle Cells; Normal, Human (ATCC® PCS-130-011)

Organism: Homo sapiens, human  /  Tissue: Bronchial-Tracheal  /  Cell Type: Smooth Muscle Cells

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Organism Homo sapiens, human
Tissue Bronchial-Tracheal
Cell Type Smooth Muscle Cells
Morphology Elongated, Spindle-shaped
Growth Properties Adherent
Biosafety Level 1 [These primary cells are not known to harbor an agent recognized to cause disease in healthy adult humans. Handle as a potentially biohazardous material under at least Biosafety Level 1 containment. Cells derived from primate lymphoid tissue may fall under the regulations of 29 CFR 1910.1030 Bloodborne Pathogens.  

ATCC recommends that appropriate safety procedures be used when handling all primary cells and cell lines, especially those derived from human or other primate material. Detailed discussions of laboratory safety procedures are provided in Laboratory Safety: Principles and Practice, 2nd ed. (ASM Press, Washington, DC) (Fleming et al., 1995) and Caputo, J.L. Biosafety procedures in cell culture. (1988) J. Tissue Culture Methods 11:223.

Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS Publication No. (CDC) 93-8395. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online at http://www.cdc.gov/biosafety/publications/bmbl5/index.htm.]

Human Material Precaution

All tissues used for isolation are obtained under informed consent and conform to HIPAA standards to protect the privacy of the donor’s personal health information. It is best to use caution when handling any human cells. We recommend that all human cells be accorded the same level of biosafety consideration as cells known to carry HIV. With infectious virus assays or viral antigen assays, even a negative test result may leave open the possible existence of a latent viral genome.

Disease Normal
Age Lot Specific
Gender Lot Specific
Ethnicity Lot Specific
Applications Applications for use include the study of angiogenesis, atherosclerosis, diabetes or vascular/pulmonary biology.
Product Format frozen 1 mL
Shipping Information Frozen
Storage Conditions -130°C
Images
Comments Human Bronchial Tracheal Smooth Muscle Cells are cryopreserved at passage 2 (cells have been isolated, plated, and expanded in culture vessels twice prior to cryopreservation) to ensure the highest viability and proliferation efficiency. These cells, when transiently transfected using TransfeX Transfection Reagent (ATCC® ACS-4005™), express high levels of GFP.

Vascular Cell Basal Medium (ATCC PCS-100-030), when supplemented with Vascular Smooth Muscle Cell Growth Kit (ATCC PCS-100-042), provides an ideal system to propagate bronchial tracheal smooth muscle cells.

Complete Growth Medium
  1. Obtain one growth kit from the freezer; make sure that the caps of all components are tight.
  2. Thaw the components of the growth kit just prior to adding them to the basal medium. It is necessary to warm the L-glutamine component in a 37°C water bath and shake to dissolve any precipitates prior to adding to the basal medium.
  3. Obtain one bottle of Vascular Cell Basal Medium (475 mL) from cold storage.
  4. Decontaminate the external surfaces of all growth kit component vials and the basal medium bottle by spraying them with 70% ethanol.
  5. Using aseptic technique, and working in a laminar flow hood or biosafety cabinet, transfer the volume of each growth kit component, as indicated in either Table 1 or 2, to the bottle of basal medium using a separate sterile pipette for each transfer.
  6. Tightly cap the bottle of complete growth medium and swirl the contents gently to assure a homogeneous solution. Do not shake forcefully to avoid foaming. Label and date the bottle.
  7. Complete growth media should be stored in the dark at 2°C to 8°C (do not freeze). When stored under these conditions, complete growth media is stable for 30 days.

 

Table 1. Vascular Smooth Muscle Cell Growth Kit Components

Component

Volume

Final Concentration

rh FGF-basic

0.5 mL

5 ng/mL

rh Insulin

0.5 mL

5 µg/mL

Ascorbic acid

0.5 mL

50 µg/mL

L-glutamine

25.0 mL

10 mM

rh EGF

0.5 mL

5 ng/mL

Fetal Bovine Serum

25.0 mL

5%

 

Antimicrobials and phenol red are not required for proliferation but may be added if desired. The recommended volume of each optional component to be added to the complete growth media is summarized in Table 2.

Table 2. Addition of Antimicrobials/Antimycotics and Phenol Red (Optional)

Component

Volume

Final Concentration

Gentamicin-Amphotericin B Solution

0.5 mL

Gentamicin: 10 µg/mL

Amphotericin B: 0.25 µg/mL

Penicillin-Streptomycin-Amphotericin B Solution

0.5 mL

Penicillin: 10 Units/mL

Streptomycin: 10 µg/mL

Amphotericin B: 25 ng/mL

Phenol Red

0.5 mL

33 µM

Volume 1 mL
Sterility Tests Bacteria and Yeast: Negative
Mycoplasma: Negative
Viral Testing
Hepatitis B: Negative
Hepatitis C: Negative
HIV: Negative
Viability ≥ 70% when thawed from cryopreservation
Population Doubling Capacity ≥ 15 in complete growth medium
C of A
Certificate of Analysis
Certificate of Analysis
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
FAQs
  1. Differentiating primary smooth muscle cells
    Yes.  ATCC primary smooth muscle cells have been tested for differentiation.  When these cells differentiate, there are visual morphological changes and increased smooth muscle alpha-ac...
    Date Updated: 4/8/2014
  2. Expressed immunophenotype for ATCC primary smooth muscle cells


    Date Updated: 4/8/2014

  3. Transfecting differentiated primary smooth muscle cells


    Date Updated: 4/8/2014

  4. ATCC primary smooth muscle cells positive for CD90


    Date Updated: 4/8/2014

  5. Primary smooth muscle cell differentiation procedure

    For primary smooth muscle differentiation we utilize the following differentiation medium:


    Date Updated: 4/8/2014