Primary Peripheral Blood CD14+ Monocytes (PBMC), Normal, Human (ATCC® PCS-800-010)

Organism: Homo sapiens, human  /  Tissue: peripheral blood  /  Cell Type: monocytes

Permits and Restrictions

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Organism Homo sapiens, human
Tissue peripheral blood
Cell Type monocytes
Morphology spherical; variable after culturing
Growth Properties suspension; variable after culturing
Biosafety Level 1  [These primary cells are not known to harbor an agent recognized to cause disease in healthy adult humans. Handle as a potentially biohazardous material under at least Biosafety Level 1 containment. Cells derived from primate lymphoid tissue may fall under the regulations of 29 CFR 1910.1030 Bloodborne Pathogens. 

ATCC recommends that appropriate safety procedures be used when handling all primary cells and cell lines, especially those derived from human or other primate material. Detailed discussions of laboratory safety procedures are provided in Laboratory Safety: Principles and Practice, 2nd ed. (ASM Press, Washington, DC) (Fleming et al., 1995) and Caputo, J.L. Biosafety procedures in cell culture. (1988) J. Tissue Culture Methods 11:223. Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS Publication No. (CDC) 93-8395. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online at http://www.cdc.gov/biosafety/publications/bmbl5/index.htm.] 

Human Material Precaution 
All tissues used for isolation are obtained under informed consent and conform to HIPAA standards to protect the privacy of the donor’s personal health information. It is best to use caution when handling any human cells. We recommend that all human cells be accorded the same level of biosafety consideration as cells known to carry HIV. With infectious virus assays or viral antigen assays, even a negative test result may leave open the possible existence of a latent viral genome.

Disease normal
Age lot specific
Gender lot specific
Ethnicity lot specific
Applications Applications for use include the study of immunology, infection, cancer, hematology, and t-cell suppression assay.
Product Format frozen 1 mL
Storage Conditions -130°C
Images
Comments CD14+ cells have a limited lifespan in culture and should only be thawed immediately prior to their intended use. ATCC does not recommend maintaining CD14+ monocytes cells in culture in the absence of application-specific growth factors.
Complete Growth Medium CD14+ cells have a limited lifespan in culture and should only be thawed immediately prior to their intended use. ATCC does not recommend maintaining CD14+ monocytes cells in culture in the absence of application-specific growth factors.
Volume 1 mL
Viral Testing
Hepatitis B: Negative
Hepatitis C: Negative
HIV(I/II): Negative
HTLV(I/II): Negative
WNV: Negative
Trypanasome: Negative
Viability ≥ 70% when thawed from cryopreservation
Population Doubling Capacity N/A
C of A
Certificate of Analysis
Certificate of Analysis
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
FAQs
  1. Human Peripheral Blood CD14+ Monocytes, ATCC® PCS-800-010™ cyopreservation media


    Date Updated: 8/18/2014

  2. Human Peripheral Blood CD14+ Monocytes, ATCC® PCS-800-010

    CD14+ monocytes are isolated from peripheral blood derived mononuclear cells by positive selection using immunomagnetic cell separation procedures.


    Date Updated: 8/18/2014
  3. Expansion/differentiation capacity of ATCC® PCS-800-010 ™ PCS-800-010 CD14+ monocytes
    ATCC primary CD14+ monocytes have been tested to differentiate into dendritic cells and macrophages, as assessed by morphology and flow cytometry, using commonly reported CD markers.  H...
    Date Updated: 8/18/2014
  4. Generation of dendritic cells (DCs) from ATCC® PCS-800-010 ™ primary CD14+ monocytes

    For dendritic cell differentiation we have successfully used the following protocol:


    Date Updated: 8/18/2014
  5. Purity of ATCC CD14+ primary monocytes
    ATCC primary CD14+ monocytes cells have been tested to have a purity of over 90% as measured by flow cytometry analysis using a monoclonal CD14 antibody.
    Date Updated: 8/18/2014
  6. Thawing Human Peripheral Blood CD14+ Monocytes, ATCC® PCS-800-010 ™

    We recommend the following thawing procedure:


    Date Updated: 8/18/2014
  7. Generation of macrophages from ATCC® PCS-800-010 ™ primary CD14+ monocytes

    For macrophage differentiation we have successfully used the following protocol:


    Date Updated: 8/18/2014
  8. Long-term culture of primary CD14+ monocytes


    Date Updated: 8/18/2014

  9. Primary CD14+ monocytes from non-normal donors
    No, at this time ATCC does not offer primary CD14+ monocytes from non-normal donors. ATCC® PCS-800-010 ™ CD14+ monocytes are from a normal donor.
    Date Updated: 8/18/2014
  10. Primary CD14+ monocytes - donor source
    No, a single cryovial of primary CD14+ monocytes only contains cells from a single donor.
    Date Updated: 8/18/2014