Primary Bone Marrow CD34+ Cells, Normal, Human (ATCC® PCS-800-012)

Organism: Homo sapiens, human  /  Tissue: bone marrow  /  Cell Type: hematopoietic stem cell

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Organism Homo sapiens, human
Tissue bone marrow
Cell Type hematopoietic stem cell
Morphology spherical; variable after culturing
Growth Properties suspension; variable after culturing
Biosafety Level 1  [These primary cells are not known to harbor an agent recognized to cause disease in healthy adult humans. Handle as a potentially biohazardous material under at least Biosafety Level 1 containment. Cells derived from primate lymphoid tissue may fall under the regulations of 29 CFR 1910.1030 Bloodborne Pathogens. 

ATCC recommends that appropriate safety procedures be used when handling all primary cells and cell lines, especially those derived from human or other primate material. Detailed discussions of laboratory safety procedures are provided in Laboratory Safety: Principles and Practice, 2nd ed. (ASM Press, Washington, DC) (Fleming et al., 1995) and Caputo, J.L. Biosafety procedures in cell culture. (1988) J. Tissue Culture Methods 11:223. Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS Publication No. (CDC) 93-8395. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online at http://www.cdc.gov/biosafety/publications/bmbl5/index.htm.] 

Human Material Precaution 
All tissues used for isolation are obtained under informed consent and conform to HIPAA standards to protect the privacy of the donor’s personal health information. It is best to use caution when handling any human cells. We recommend that all human cells be accorded the same level of biosafety consideration as cells known to carry HIV. With infectious virus assays or viral antigen assays, even a negative test result may leave open the possible existence of a latent viral genome.

Disease normal
Age lot specific
Gender lot specific
Ethnicity lot specific
Applications Applications for use include the study of stem cell biology, hematology, transplantation, and oncology.
Product Format frozen 0.50 mL
Storage Conditions liquid nitrogen vapor phase
Comments Cluster of differentiation 34 (CD34) is a sialomucin-family protein that is expressed on the surface of blood, endothelial, and bone marrow-derived progenitor cells. While the functions of CD34 are not well understood, there is evidence that the protein is involved in maintaining progenitor cells in an undifferentiated state.

The ATCC maintains CD34+ bone marrow-derived and CD34+ cord blood-derived cells which may be used to support physiologically relevant research in regenerative medicine, transplant studies, stem cell biology, hematology, and oncology. These pluripotent stem cells are isolated by immunomagnetic positive selection. In addition, the ATCC Cell Systems Laboratory has conducted in-depth characterization of the cells including differentiation into all three cell lineages.

Complete Growth Medium CD34+ cells have a limited lifespan in culture and should only be thawed immediately prior to their intended use. ATCC does not recommend maintaining CD34+ cells in culture in the absence of application-specific growth factors.
Volume 0.50 mL
Viral Testing
Hepatitis B: Negative
Hepatitis C: Negative
HIV(I/II): Negative
HTLV(I/II): Negative
WNV: Negative
Trypanasome: Negative
Viability ≥ 70% when thawed from cryopreservation
C of A
Certificate of Analysis
Certificate of Analysis
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
FAQs
  1. Post-thaw yield and viability of ATCC® PCS-800-012 ™ Human Primary Bone Marrow CD34+ cells

    We recommend the following thawing procedure:


    Date Updated: 8/27/2014
  2. Isolation of ATCC® PCS-800-012 ™, Human Primary Bone Marrow CD34+ cells
    CD34+ cells are isolated using positive immunomagnetic cell separation from the bone marrow.
    Date Updated: 8/27/2014
  3. Long term culture of ATCC® PCS-800-012
    CD34+ cells have a limited lifespan in culture and should only be thawed immediately prior to their intended use. ATCC does not recommend maintaining CD34+ cells in culture in the absence of appl...
    Date Updated: 8/27/2014
  4. Purity of ATCC® PCS-800-012, Human Primary Bone Marrow CD34+ cells
    ATCC CD34+ cells have been tested to have a purity of over 90% as measured by flow cytometry with a monoclonal CD34 antibody.
    Date Updated: 8/27/2014
  5. Cryopreservative used with PCS-800-012
    ATCC CD34+ cells are cryopreserved in CryoStor® CS10 Freeze Medium from BioLife Solutions.
    Date Updated: 8/27/2014
  6. CD34 positive primary cells from non-normal donors

    No, at this time ATCC does not offer CD34+ cells from non-normal donors. The ATCC® PCS-800-012 ™, Human Primary Bone Marrow CD34+ cells are from normal donor sources.


    Date Updated: 8/27/2014
  7. Data on the percentage of CD34 positive cell subtypes
    No. ATCC does not routinely test for markers other than CD34. The makeup of specific CD34+ subtypes contained within each vial of cells will vary greatly due to donor and lot variability.
    Date Updated: 8/27/2014
  8. Expansion/differentiation capacity of ATCC® CD34+ primary cells
    ATCC CD34+ primary cells have been tested for their ability to differentiate into myeloid, erythroid and megakaryocyte cells, as assessed by flow cytometry using commonly reported CD markers.&nbs...
    Date Updated: 8/27/2014
  9. Protocol for the expansion/differentiation of CD34+ primary cells


    Date Updated: 8/27/2014