HCM-CSHL-0061-C18 (ATCC® PDM-5)

Organism: Homo sapiens, human  /  Tissue: sigmoid colon  /  Disease: primary adenocarcinoma

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Organism Homo sapiens, human
Tissue sigmoid colon
Product Format frozen 1.0 mL
Morphology organoid
Culture Properties embedded 3D culture
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease primary adenocarcinoma
Age See associated clinical data for patient profile information, if available.
https://portal.gdc.cancer.gov/  
https://portal.hcmi.cancercollaboratory.org/

Gender female
Ethnicity See associated clinical data for patient profile information, if available.
https://portal.gdc.cancer.gov/  
https://portal.hcmi.cancercollaboratory.org/

Applications Research purposes, compound screening
Storage Conditions liquid nitrogen vapor phase
Images
Clinical Data ICD-10-CM code: C18, malignant neoplasm of colon
https://portal.hcmi.cancercollaboratory.org/

Comments Next-generation cancer model from the Human Cancer Models Initiate (HCMI). Refer to the following websites for additional information on this model including protocols, clinical information, and bioinformatics data.

https://ocg.cancer.gov/programs/hcmi/resources
https://portal.gdc.cancer.gov/  
https://portal.hcmi.cancercollaboratory.org/

Complete Growth Medium

To prepare the complete medium for this organoid please refer to the Organoid Media Formulation #1.

Subculturing

Initiating culture from frozen vials: For a brief overview of the thawing procedure see our quickstart guide Thawing Cryopreserved Human Organoids.

Seeding density: 0.25 - 1 x 106/ viable cells in 100 µL of ECM per well of a 6-well plate. Alternatively, split at 1:2-1:4 every 7-10 days. For example, collect organoids from 100 µL of extracellular matrix (ECM) from a single well of a 6-well plate and re-seed into 2-4 wells of a 6-well plate in 100 µL ECM per well. 

Media renewal: Perform a complete medium change every 2-3 days. Include 10 µM ROCK Inhibitor Y-27632 (ATCC ACS-3030) in medium for the first 2-3 days following subculture. 

For a brief overview of the subculture and expansion of organoids see our quickstart guide Subculture and Expansion of Human Organoids Protocol.

For more details on the handling and culture of organoids see our methods paper in Current Protocols in Cell Biology

Cryopreservation

We recommend cryopreserving this model in ATCC Stem Cell Freezing Media (ATCC ACS-6000). For a brief overview of the cryopreservation procedure for organoids see our quickstart guide Organoid Cryopreservation Protocol.

For more details on the handling and culture of organoids see our methods paper in Current Protocols in Cell Biology.

Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
Cells per Vial ≥ 1 x 106
Volume 1.0 mL
Sterility Tests Bacteria and yeast: No growth
Mycoplasma: No growth
Viral Testing Hepatitis B: None detected
Cytomegalovirus: None detected
Human immunodeficiency virus: None detected
Epstein-Barr virus: None detected
Human papillomavirus: None detected
Name of Depositor Cold Spring Harbor Laboratory
Year of Origin 2017
Basic Documentation
Other Documentation
Restrictions

This material is only distributed under the additional terms of a fully signed HUB Addendum. Prior to shipment, all customers must sign and return the HUB Addendum.