Ectromelia virus (ATCC® VR-1374)

Classification: Poxviridae, Orthopoxvirus  /  Product Format: frozen

Permits and Restrictions

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Classification Poxviridae, Orthopoxvirus
Agent Ectromelia virus
Strain Moscow
Applications
1982. Mousepox, (in) The Mouse in Biomedical Research: Vol.
Requests for the agent must carry a signed statement assuming all risk and responsibility for handling and use.
Biosafety Level 2
Isolation
tissue, animal
Product Format frozen
Storage Conditions -70°C or colder (or colder)
Comments
Inadvertent introduction of Ectromelia virus into mouse colonies has caused major outbreaks in US animal colonies. The depositor recommends that animal care and biosafety management at your institution be consulted prior to initiating work with this agent. Requests for the agent must carry a signed statement assuming all risk and responsibility for handling and use. For additional information see: Fenner. 1982. Mousepox, (in) The Mouse in Biomedical Research: Vol. II. Diseases, (ed) Foster et al., Academic Press.
Effect on Host
Yes, in vitro effects: cytopathic effects, refractile rounding
CPE, refractile rounding; death in mice
Yes, in vivo effects: death of mouse
Growth Medium Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 98%; fetal bovine serum, 2%
Recommended Host

Recommended Host:  BS-C-1 (ATCC® CCL-26TM)

Growth Conditions
Atmosphere: 5% CO2 in air recommended
Temperature: 37.0°C
Duration: 1-7 days; For best results cells should be 24 to 48 hours old and 80-90% confluent (not 100% confluent).
Cryopreservation
Storage temperature: -70°C
Effect on Host
Yes, in vitro effects: cytopathic effects, refractile rounding
CPE, refractile rounding; death in mice
Yes, in vivo effects: death of mouse
Name of Depositor RM Buller
Special Collection ATCC
Source
tissue, animal
References

Chen W, et al. Restricted replication of ectromelia virus in cell culture correlates with mutations in virus-encoded host range gene. Virology 187: 433-442, 1992. PubMed: 1546448

or colder

spleen homogenate from a BALB/c mouse 6 days post-infection

or colder

Cross References

Nucleotide (GenBank) : AF012825 Ectromelia virus envelope glycoprotein C1R (C1R), C2R (C2R), C3R (C3R), IFN-gamma binding protein C4R (C4R), C5R (C5R), protein kinase C6R (C6R), serpin C7R (C7R), C8R (C8R), IL-1 beta binding protein C9R (C9R), C10L (C10L), C11R (C11R), IFN-...

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Basic Documentation