Human herpesvirus 5 (ATCC® VR-1788)

Classification: Herpesviridae, Cytomegalovirus  /  Product Format: frozen 1 mL per vial

Classification Herpesviridae, Cytomegalovirus
Agent Human herpesvirus 5
Strain ATCC-2011-8
Common Name Human cytomegalovirus
Biosafety Level 2
Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Images VR-1788 images
Comments Virus primarily cell-associated. Cryopreserve virus in 7% DMSO (ATCC® 4-X™).
Effect on Host
CPE, rounding and clustering
Recommended Host
MRC-5 (ATCC® CCL-171™)
Growth Conditions Temperature: 37°C
Recommendations for Infection: This product is produced by co-cultivation of virus with fresh host cells. Prepare a bulk cell suspension the day of inoculation. Seed culture vessels at 1.3-1.5 x 104 cells per cm2. Calculate the volume of virus needed to achieve an optimal MOI (e.g. 0.1) and then dilute virus in virus growth medium to prepare the virus inoculum. Add virus inoculum to culture vessels. Incubate for 24 hours at 37°C in a humidified 5% CO2 atmosphere. Aspirate virus growth medium to remove any traces of DMSO and then add fresh virus growth medium to cultures. Continue incubation.
Incubation: 14 days
Effect on Host
CPE, rounding and clustering
Name of Depositor ATCC®
Source Clinical patient positive for CMV, Virginia, July 2011