Human Coxsackievirus A6 (ATCC® VR-1801)

Classification: Picornaviridae, Enterovirus, Human enterovirus A  /  Product Format: frozen 1.0 mL per vial

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Classification Picornaviridae, Enterovirus, Human enterovirus A
Agent Human Coxsackievirus A6
Strain Gdula
Biosafety Level 2
Product Format frozen 1.0 mL per vial
Storage Conditions -70°C
Images ATCC VR-1801 Photomicrographs
Comments Derived by adaptation of primary cell culture product ATCC® VR-165™ to RD cells (ATCC® CCL-136™).  ATCC® VR-165™ and ATCC® VR-1801 have not been compared for sequence of infectivity in primary cell culture and tissue culture.
Effect on Host
CPE, cell rounding and death
Recommended Host
RD cells (ATCC® CCL-136™)
Growth Conditions Temperature: 37°C
Recommendations for Infection: For best results, infection should be performed on a 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2), optimized for propagation (e.g. MOI 0.01-0.1 recommended). Wash monolayer with DPBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 2-4 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer.
Effect on Host
CPE, cell rounding and death
Name of Depositor HA Wenner
Source Stool from 5-year-old child with sore throat, fever and leg muscle spasm, New York, 1949.
References

Dalldorf G. The coxsackie virus group. Ann. N.Y. Acad. Sci. 56: 583-586, 1953. PubMed: 13139263

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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