Human Coxsackievirus A6 (ATCC® VR-1801)

Classification: Picornaviridae, Enterovirus, Human enterovirus A  /  Product Format: frozen 1.0 mL per vial

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Classification Picornaviridae, Enterovirus, Human enterovirus A
Agent Human Coxsackievirus A6
Strain Gdula
Biosafety Level 2

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen 1.0 mL per vial
Storage Conditions -70°C
Images ATCC VR-1801 Photomicrographs
Comments Derived by adaptation of primary cell culture product ATCC® VR-165™ to RD cells (ATCC® CCL-136™).  ATCC® VR-165™ and ATCC® VR-1801 have not been compared for sequence of infectivity in primary cell culture and tissue culture.
Effect on Host
CPE, cell rounding and death
Recommended Host
RD cells (ATCC® CCL-136™)
Growth Conditions Temperature: 37°C
Recommendations for Infection: For best results, infection should be performed on a 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2), optimized for propagation (e.g. MOI 0.01-0.1 recommended). Wash monolayer with DPBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 2-4 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer.
Effect on Host
CPE, cell rounding and death
Name of Depositor HA Wenner
Source Stool from 5-year-old child with sore throat, fever and leg muscle spasm, New York, 1949.
References

Dalldorf G. The coxsackie virus group. Ann. N.Y. Acad. Sci. 56: 583-586, 1953. PubMed: 13139263

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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