Influenza A virus (H3N2) (ATCC® VR-1811)

Classification: Orthomyxoviridae, Influenzavirus A  /  Product Format: frozen

Permits and Restrictions

View Permits

Classification Orthomyxoviridae, Influenzavirus A
Agent Influenza A virus (H3N2)
Strain A/Virginia/ATCC6/2012
Biosafety Level 2

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen
Storage Conditions -70°C or colder
Images ATCC VR-1811 images
Comments This item was isolated in cell culture by three passages in MDCK cells.
Effect on Host
CPE, rounding and sloughing
Recommended Host
MDCK cells (ATCC® CCL-34™)
Growth Conditions Temperature: 35°C
Recommendations for Infection: For best results, infection should be performed on a 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2). Wash monolayer three times with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 35°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 2-3 days at 35°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer.
Effect on Host
CPE, rounding and sloughing
Source Clinical specimen from a patient in Virginia, 2012.
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation