Francisella persica Larson (ATCC® VR-1853)

Classification: Thiotrichales, Francisellaceae  /  Product Format: frozen 1.0 mL per vial

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Classification Thiotrichales, Francisellaceae
Deposited As Wolbachia persica Suitor and Weiss
Agent Francisella persica Larson
Strain Original
Applications Vector-borne pathogens research
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen 1.0 mL per vial
Storage Conditions -70°C or colder
Images VR-1853 images
Comments Derived by adaptation of egg culture product ATCC® VR-331™ to Aedes albopictus clone C6/36 cells (ATCC® CRL-1660™). ATCC® VR-331™ and ATCC® VR-1853™ have not been compared for sequence or infectivity in egg culture and tissue culture.
Effect on Host
CPE, refractile rounding and sloughing
Recommended Host
Aedes albopictus clone C6/36 cells (ATCC® CRL-1660™)
Growth Conditions

Temperature: 28°C

Recommendations for Infection: Plate cells 18-24 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g., 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g., 0.01). Adsorb 1-2 hours at 28°C in a humidified, air atmosphere incubator. End adsorption by adding virus growth medium.

Incubation: 6-8 days at 28°C in a humidified, air atmosphere incubator, until CPE is progressed through 80% of the monolayer.

Effect on Host
CPE, refractile rounding and sloughing
Name of Depositor E Weiss, Naval Medical Research Center
Source Malpighian tubules of ticks, Argas persicus (Oken) (now Argas (Persicargas) arboreus), removed from buff-backed heron (Bubulcus ibis), U.A.R.
References

Suitor EC, Weiss E. Isolation of a rickettsialike microorganism (Wolbachia persica n. sp.) from Argas persicus (Oken). J. Infect. Dis. 108: 95-106, 1961.

Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.

Larson MA, et al. Reclassification of Wolbachia persica as Francisella persica comb. nov. and emended description of the family Francisellaceae. Int. J. Syst. Evol. Microbiol. 66(3): 1200-1205, 2016. PubMed: 26747442

Cross References

Nucleotide (GenBank) : M21292 Wolbachia persica 16S ribosomal RNA.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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