Influenza B virus (Victoria Lineage) (ATCC® VR-1931)

Classification: Orthomyxoviridae, Influenzavirus B  /  Product Format: frozen 0.5 mL per vial

Classification Orthomyxoviridae, Influenzavirus B
Deposited As Influenza B virus
Agent Influenza B virus (Victoria Lineage)
Strain B/Florida/78/2015 (propagated in cell culture)
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen 0.5 mL per vial
Storage Conditions -70°C or colder
Effect on Host
CPE, refractile rounding and sloughing
Recommended Host
MDCK cells (ATCC® CCL-34™)
Growth Conditions

Temperature: 35°C

Recommendations for Infection: Plate cells 18-24 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1 to 0.01). Adsorb 1-2 hours at 35°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium.

Incubation: 2-3 days at 35°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 75% of the monolayer.

Effect on Host
CPE, refractile rounding and sloughing
Name of Depositor Florida Department of Health
Source Isolated from a human in Florida, USA on November 09, 2015
References

Influenza Research Database – Details for B/Florida/78/2015