Caviid herpesvirus 2 (ATCC® VR-682)

Classification: Herpesviridae, Unassigned  /  Product Format: frozen 1 mL per vial

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Classification Herpesviridae, Unassigned
Agent Caviid herpesvirus 2
Strain 22122
Biosafety Level 2
Isolation
Submaxillary glands from a guinea pig
Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Effect on Host CPE, refractile rounding and sloughing
Recommended Host Guinea pig lung (ATCC CCL-158)
Growth Conditions Temperature: 37°C

Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 90-95% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified atmosphere, rocking every 20-30 minutes to redistribute inoculum.  End adsorption by adding virus growth medium.

Incubation: 9-21 days
Effect on Host CPE, refractile rounding and sloughing
Name of Depositor MJ Collins, Jr., Microbiological Associates, Inc.
Source
Submaxillary glands from a guinea pig
References

Hartley JW, et al. Serial propagation of the guinea pig salivary gland virus in tissue culture. Proc. Soc. Exp. Biol. Med. 96: 281-285, 1957. PubMed: 13485080

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation