Canine parvovirus (ATCC® VR-953)

Classification: Parvoviridae, Parvovirus, Feline panleukopenia virus  /  Product Format: frozen 1 mL per vial

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Classification Parvoviridae, Parvovirus, Feline panleukopenia virus
Agent Canine parvovirus
Strain C-780916
Applications
Antigenically indistinguishable from feline panleukopenia virus and mink enteritis virus, but it has a broader host range and different HA spectrum, especially as regards pH conditions.
Hemagglutinates swine, Rh+ monkey & cat RBC at pH 6.0-7.5. On passage, add virus 2 hrs after setting host cells.
Antigenically indistinguishable from feline panleukopenia virus and mink enteritis virus, but it has a broader host range and different HA spectrum, especially as regards pH conditions.
Biosafety Level 2
Isolation
Feces of dog with hemorrhagic enteritis
Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Comments
Non-pathogenic for neonatal pups. Antigenically indistinguishable from Feline panleukopenia virus and Mink enteritis virus, but has broader host range and different HA spectrum, especially in regards to pH conditions. Hemagglutinates porcine, Rh+ monkey, and feline red blood cells at pH 6.0-7.5. May fail to immunize dogs if passaged 8-10 times in feline cells.
Effect on Host
CPE, refractile cells, cell rounding and sloughing
Recommended Host
A-72 (ATCC CRL-1542)
Growth Conditions Temperature: 35°C
Recommendations for Infection: Plate cells 2-4 hours prior to infection and infect when cultures are 30-40% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 35°C in a humidified atmosphere, rocking every 15 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 3-9 days
Effect on Host
CPE, refractile cells, cell rounding and sloughing
Name of Depositor LE Carmichael, New York State College of Veterinary Medicine, Cornell University
Source Feces of dog with hemorrhagic enteritis, 1978
References

Appel MJ, et al. Isolation and immunisation studies of a canine parco-like virus from dogs with haemorrhagic enteritis. Vet. Rec. 105: 156-159, 1979. PubMed: 516347

Carmichael LE, et al. A modified live canine parvovirus strain with novel plaque characteristics. I. Viral attenuation and dog response. Cornell Vet. 71: 408-427, 1981. PubMed: 7318443

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Basic Documentation