PSN-1 (ATCC® CRM-CRL-3211)

Organism: Homo sapiens, human  /  Tissue: pancreas  /  Disease: adenocarcinoma

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Organism Homo sapiens, human
Tissue pancreas
Product Format frozen
Morphology epithelial-like
Culture Properties adherent
Biosafety Level 1  [This is Certified Reference Material produced under an ISO Guide 34:2009 accredited process. This item has been tested for KRAS mutation: Homozygous DNA Change: c.34G>C (correlates to protein sequence p.G12R).]
Disease adenocarcinoma
Intended Use

Certified Reference Material produced under an ISO Guide 34:2009 accredited process. 


    ACLASS Accredited Reference Material Producer AR-1384

Storage Conditions liquid nitrogen vapor phase
Comments

Certificates of Analysis are available electronically at www.atcc.org, or by hardcopy upon request.

Certified Reference Material produced under an ISO Guide 34:2009 accredited process.

Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: heat-inactivated fetal bovine serum to a final concentration of 10%.
Subculturing Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with Ca2+/Mg2+ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.025% (w/v) Trypsin-EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 1.0 to 2.0 mL of 0.025% Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Transfer cell suspension to a centrifuge tube and spin at approximately 125 x g for 5 to 10 minutes. Discard supernatant.
  6. Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels. An inoculum of 1.5 X 104 to 3.0 X 104 viable cells/cm^2 is recommended.
  7. Incubate cultures at 37°C. 

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze Medium: Complete growth medium supplemented with an additional 10% heat inactivated fetal bovine serum and 10% (v/v) DMSO
Storage Temperature: Liquid nitrogen vapor phase
Culture Conditions
Temperature: 35°C to 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
FAQ's
  1. Agreement to transfer to a CRO


    Date Updated: 1/2/2013