Clostridium difficile (Hall and O'Toole) Prevot (ATCC® 51695-FZ)

Strain Designations: BDMS 18 AN  /  Biosafety Level: 2

Strain Designations BDMS 18 AN
Application
Quality control strain
Quality control strain for Clostridium difficile agar
Toxin B production confirmed by cytotoxicity assay using Vero cells (Cell line # CCL-81).
Enteric Research
Biosafety Level 2
Product Format frozen
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol® no
Genotype Toxinotype: 0
Comments
Presence of tcdA and tcdB genes confirmed by PCR. RefLemee L, et al. Multiplex PCR targeting tpi (triose phosphate isomerase), tcdA (Toxin A), and tcdB (Toxin B) genes for toxigenic culture of Clostridium difficile. J. Clin. Microbiol. 42(12): 5710-5714, 2004. PubMed: 15583303
Binary toxin gene cdtB not amplified by PCR
Toxinotype 0
Ribotype 001
Toxin B production confirmed by cytotoxicity assay using Vero cells (Cell line # CCL-81).
Medium Medium 2107: Modified Reinforced Clostridial Agar/Broth Medium (pre-reduced)
Medium 260: Trypticase soy agar/broth with defibrinated sheep blood
Growth Conditions Temperature: 37°C
Atmosphere: Anaerobic gas mixture, 80% N2-10% CO2-10% H2
Genotype Toxinotype: 0
Name of Depositor Becton Dickinson Microbiology Systems
Special Collection NCRR Contract
Chain of Custody ATCC <-- Becton Dickinson Microbiology Systems <-- J Aswell <-- Johns Hopkins Univ. Hosp. Lab
References

Lemee L, et al. Multiplex PCR targeting tpi (triose phosphate isomerase), tcdA (Toxin A), and tcdB (Toxin B) genes for toxigenic culture of Clostridium difficile. J. Clin. Microbiol. 42(12): 5710-5714, 2004. PubMed: 15583303

Rupnik, M et al. A novel toxinotyping scheme and correlation of toxinotypes with serogroups of Clostridium difficile isolates. J. Clin. Microbiol. 36(8): 2240-2247, 1998. PubMed: 9665999

Fawley WN, Wilcox MH; on behalf of the Clostridium difficile Ribotyping Network for England and N. Ireland (CDRN). An enhanced DNA fingerprinting service to investigate potential Clostridium difficile infection case clusters sharing the same PCR-ribotype. J. Clin. Microbiol. 49(12): 4333-4337, 2011. PubMed: 21956986