Fetal Bovine Serum (ATCC® 30-2021)

Product Format frozen 100 mL
Storage Conditions Store fetal bovine serum at -20°C or colder. Do not store frozen fetal bovine serum at temperatures above -20°C for any length of time. Avoid repeated freeze-thaws by dispensing and storing fetal bovine serum in aliquots. Fetal bovine serum is supplied in PETG plastic bottles.
Applications

Triple filtered through 0.1 μm filters. Each lot of fetal bovine serum is tested for sterility and for the ability to support the growth of several different cell lines using both sequential growth curves and plating efficiencies. Fetal bovine serum is manufactured from fetal bovine blood collected in USDA-inspected abattoirs located in the United States.

Biosafety Level N/A
Quality Control Specifications

Sterility tests are performed on each lot of fetal bovine serum using current USP and EP methods. Specific tests for contaminants are listed below in the Specification Certificate.  ATCC also checks each lot for its ability to support the growth of several different cell lines. Additionally, this lot of serum is tested for its ability to support the growth and differentiation of embryonic stem (ES) cells. Plating efficiency, colony morphology, and the expression of at least five markers of differentiation are determined for undifferentiated cells and cells induced to form embryoid bodies (EB) through full differentiation. The results are compared with cells grown with a control lot of serum.

Origin
This fetal bovine serum is manufactured from fetal bovine blood collected in USDA-inspected abattoirs located in the United States.


Test*

Specification

Sterility Testing (Current USP)

Bacteria and Fungi

No growth

Virus Testing (9 CFR 113.53)

Fluorescent antibody

Bluetongue

Bovine Adenovirus

Bovine Parvovirus

Bovine Respiratory Syncytial Virus

Bovine Diarrhea Virus

Rabies

Reovirus

Cytopathogenic Agents (e.g. IBR)

Hemadsorbing Agents (e.g., PI3)

Not detected

 

 

 

 

 

 

Mycoplasma Testing

Large volume, direct culture                       Hoechst DNA stain                   

Pass

Endotoxin (Limulus Amebocyte Lysate -Gel Clot)

≤ 10 EU/mL

Hemoglobin (Spectrophotometric)

Report result

Growth Promotion

Pass

*Please consult the Certificate of Analysis for lot-specific test results.