L-Glutamine Solution, 200 mM (ATCC® 30-2214)

Product Format frozen 100 mL
Storage Conditions Store L-glutamine solution frozen at -20°C or colder. Avoid repeated freeze-thaws by dispensing and storing in aliquots.
Applications L-Glutamine is an essential amino acid required by virtually all mammalian and insect cells grown in culture.

L-Glutamine is also more labile in cell culture solutions than other amino acids. The rate and extent of L-glutamine degradation are related to storage temperatures, age of the product, and pH.

All cell culture media are formulated to contain appropriate amounts of L-glutamine to support cell growth well beyond the expiration date of the media. However, if L-glutamine is suspected to be a limiting factor during cell culture, a simple test of 'spiking' the medium with a small amount of L-glutamine will determine whether or not more is required. Simply add a small amount of L-glutamine (~ 2 mM final concentration) to the culture medium. If the cell growth rate increases, L-glutamine is most likely deficient and more should be added.
Comments

Added as a supplement to cell culture media. L-Glutamine is an essential amino acid required by virtually all mammalian and insect cells grown in culture. L-Glutamine is also more labile in cell culture solutions than other amino acids. The rate and extent of L-glutamine degradation are related to storage temperatures, age of the product, and pH. 

Note: L-glutamine degradation results in the build-up of ammonia which can have a deleterious effect on some cell lines. Use caution when adding more L-glutamine than called for in the original medium formulation (2 to 4 mM is standard). For most cell lines, ammonia toxicity is more critical for cell viability than L-glutamine limitation.

For more information, please contact ATCC Technical Service.

Biosafety Level 1
Quality Control Specifications Lot specific results are available in the Certificate of Analysis, which is available at www.atcc.org or by contacting ATCC technical service.

Test

Specification

Sterility Testing (USP XXIV)

No growth

Osmolality

400 to 500 mOsm/kg

pH

5.0 to 6.0

Mycoplasma

Tested Negative

Endotoxin (1:50)

0.25 EU/mL

Cell Lines

HEp-2 (CCL-23)

NG108-15 (HB-12317)

EB-3 (CCL-85)

Pass

Pass

Pass