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Geobacillus stearothermophilus (Donk) Nazina et al.

7953-MINI-PACK

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ATCC® 7953-MINI-PACK™ consists of 6 ready-to-use vials of ATCC® 7953™ frozen in 200 µL of glycerol stock, eliminating the need to rehydrate and culture the strain prior to use. Each vial is provided with a 2-D barcode for easy storage and tracking, as well as peel-off labels for fast and reliable recordkeeping.
Product category
Bacteria
Product type
Extremophile
Type strain
No
Genome sequenced strain
Yes
Product format
Frozen
Shipping information
6 ready-to-use vials in glycerol stock
Storage conditions
-70°C or colder
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Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information

General

Specific applications
ATCC® 7953-MINI-PACK™ consists of 6 ready-to-use vials of ATCC® 7953™ frozen in 200 µL of glycerol stock, eliminating the need to rehydrate and culture the strain prior to use. Each vial is provided with a 2-D barcode for easy storage and tracking, as well as peel-off labels for fast and reliable recordkeeping. Developed by the leaders in microbial cultivation and preservation, ATCC® Minis provide a convenient, ready-to-use solution for handling quality control strains. ATCC® Minis are authenticated and backed by ATCC polyphasic testing – ensuring the same consistent and reliable reference materials you’ve come to trust for ATCC Genuine Cultures®. It is easy to ensure the quality of your products with ATCC® Minis – just open, plate, and go!

Gas sterilization control
Steam sterilization control
Sterility assurance
Sterilization control
Pharmaceutical and Personal Care
Preceptrol
No

Characteristics

Extremophile type
Thermophile
Comments
This strain is recommended by ATCC for use in the tests described in military specification MIL S-36586A and U.S. Pharmacopeia, 23rd rev., <1211>, pp. 1976-1981, 1995 where only the taxon is specified.
Stated to grow at 35°C
The ATCC lab was unable to verify growth at 35°C.

Handling information

Medium
Temperature
55°C
Atmosphere
Aerobic
Handling procedure
Frozen mini-cryovials packed in dry ice should either be thawed immediately for use or stored at or below
-70°C until the expiration date printed on the label. Storage at -20°C is acceptable for up to 1 year.
  1. Thaw the bacterial strain upright using gentle agitation in a 25°C to 30°C water bath. Thawing will be rapid; approximately 2-3 minutes or until all ice crystals have melted.
  2. Immediately after thawing, wipe down the mini-cryovial with 70% ethanol and aseptically transfer the entire contents to a 5-6 mL tube of #3 broth or directly inoculate a #3 plate and/or #3 agar slant.
  3. Discard the empty vial. Do not refreeze any unused portion as it will result in a loss of viability.
  4. Incubate at 55°C for 24 hours.
Handling notes
Growth is light-moderate in broth and heavy on agar.

Colony variation may be observed and is typical for this strain.

Depositor stated that this strain grows on agar at 35°C, but this was not verified by ATCC® laboratories.

Additional information on this culture is available on the ATCC® web site at www.atcc.org. 

Quality control specifications

Verification method
Whole-genome Sequencing

History

Deposited as
Bacillus stearothermophilus Donk
Depositors
CP Hegarty
Special collection
ATCC® Minis
Cross references
GenBank L13418 Bacillus stearothermophilus maltose permease (malA) gene, complete cds.
GenBank M16814 B.stearothermophilus small acid-soluble spore protein (B-type SASP) gene, complete cds.
GenBank M86508 Bacillus stearothermophilus proton glutamate symport protein (gltP) gene, complete cds.
GenBank M13061 B.stearothermophilus SASP-1 gene encoding a small acid-soluble spore protein, complete cds.
GenBank AF184067 Bacillus stearothermophilus MetS (metS) gene, partial cds; transition state regulator (abrB) gene, complete cds; and yabC pseudogene, partial sequence.
GenBank U18943 Bacillus stearothermophilus mannitol transport protein (MtlA), putative transcriptional regulator (MtlR), mannitol enzyme IIA (MtlF) and mannitol-1-phosphate dehydrogenase (MtlD) genes, complete cds.

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

Frequently Asked Questions

References

Curated Citations

Smith NR, et al. Aerobic spore forming bacteria. US Dep Agric Monogr 16: 1-148, 1952.

Arch. Biochem. Biophys. 54: 154-161, 1955.

British Pharmacopoeia Commission Methods of sterilization. London, UK:British Pharmacopoeia Commission;British Pharmacopoeia Appendix XVIII, 2003

European Pharmacopoeia Commission Biological indicators of sterilization. Strasbourg, France:European Pharmacopoeia Commission;European Pharmacopoeia EP 5.1.2, 1997

U.S. Pharmacopeia USP Monographs: Biological Indicator for Steam Sterilization, Paper Carrier. Rockville, MD:U.S. Pharmacopeia;USP USP34-NF29, 2011

View All Curated Citations for this Product

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