Escherichia coli (Migula) Castellani and Chalmers (ATCC® BAA-181)

Strain Designations: CDC 1999-3249  /  Type Strain: no  /  Biosafety Level: 2

Classification Enterobacteriaceae, Escherichia
Strain Designations CDC 1999-3249
Application
Produces Shiga toxin 1
Produces Shiga toxin 2
Produces verocytotoxin 1
Produces verocytotoxin 2
Enteric Research
Food testing
Isolation Stool from a human with HUS, South Dakota, 1999
Type Strain no
Biosafety Level 2
Antigenic Properties Serotype O111:H8
Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol® no
Type Strain no
Antigenic Properties Serotype O111:H8
Comments This strain was confirmed by PCR to carry the eae, stx1, and stx2 genes (stx1+, stx2+, eae+).
Toxigenic

Growth on Novobiocin (10 mg/L)
Growth on Tellurite (0.8 mg/L)
Medium Medium 18: Trypticase Soy Agar/Broth
Growth Conditions
Temperature: 37°C
Atmosphere: Aerobic
Name of Depositor NA Strockbine
Special Collection NCRR Contract
Isolation Stool from a human with HUS, South Dakota, 1999
References

Nancy A Strockbine, personal communication