BS-C-1 (ATCC® CCL-26)

Organism: Cercopithecus aethiops  /  Tissue: kidney  /  Disease: Normal

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Organism Cercopithecus aethiops
Tissue kidney
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease Normal
Applications
This cell line is a suitable transfection host and may be used for the detection of viruses.
Storage Conditions liquid nitrogen vapor phase
Karyotype Chromosome Frequency Distribution 100 Cells: 2n = 60. One large submetacentric marker chromosome. Most of the cells contained a chromosome with a secondary constriction and many of the cells contained dicentric chromosomes.
Genes Expressed
keratin
Cellular Products
keratin
Virus Susceptibility Simian virus 40 , Simian virus 40
Human poliovirus 1
Vesicular stomatitis, Orsay (Indiana)
Vesicular stomatitis, Glasgow (Indiana)
Comments
The cells are positive for keratin by immunoperoxidase staining.

African green monkey kidney cell line suitable for transfection by SV40 vectors.

Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing

Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

  1. Remove and discard culture medium. 
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach.  Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. 
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Two to three times weekly
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Culture Conditions
Temperature: 37°C
Name of Depositor HE Hopps
References

Hopps, H. E., et al. Biologic characteristics of the continuous kidney cell line derived from the African green monkey. J. Immunol. 91: 416-424, 1963. PubMed: 14071033

American Public Health Association. Compendium of methods for the microbiological examination of foods. 3rd ed.Washington, DC: American Public Health Association; 1992.

Berson JF, et al. A seven-transmembrane domain receptor involved in fusion and entry of T-cell-tropic human immunodeficiency virus tyep 1 strains. J. Virol. 70: 6288-6295, 1996. PubMed: 8709256

Jelachich ML, Lipton HL. Theiler's murine encephalomyelitis virus kills restrictive but not permissive cells by apoptosis. J. Virol. 70: 6856-6861, 1996. PubMed: 8794327

Schmidt NJ, et al. The sensitivity of grivet monkey kidney cell line BS-C-1 for propagation and isolation of certain human viruses. Am. J. Public Health 54: 1522-1530, 1964.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Hopps, H. E., et al. Biologic characteristics of the continuous kidney cell line derived from the African green monkey. J. Immunol. 91: 416-424, 1963. PubMed: 14071033

American Public Health Association. Compendium of methods for the microbiological examination of foods. 3rd ed.Washington, DC: American Public Health Association; 1992.

Berson JF, et al. A seven-transmembrane domain receptor involved in fusion and entry of T-cell-tropic human immunodeficiency virus tyep 1 strains. J. Virol. 70: 6288-6295, 1996. PubMed: 8709256

Jelachich ML, Lipton HL. Theiler's murine encephalomyelitis virus kills restrictive but not permissive cells by apoptosis. J. Virol. 70: 6856-6861, 1996. PubMed: 8794327

Schmidt NJ, et al. The sensitivity of grivet monkey kidney cell line BS-C-1 for propagation and isolation of certain human viruses. Am. J. Public Health 54: 1522-1530, 1964.