SCC-PSA1 (ATCC® CRL-1535)

Organism: Mus musculus, mouse  /  Cell Type: fibroblast  /  Disease: pluripotent teratocarcinoma

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Organism Mus musculus, mouse
Cell Type fibroblast
Product Format frozen
Culture Properties adherent
Biosafety Level 1
Disease pluripotent teratocarcinoma
Gender male
Strain 129/Sv
Applications
The cells can be maintained in an undifferentiated state by frequent subculture and the use of a fibroblast feeder cell layer (see ATCC CRL-1503).
In the absence of a feeder layer, the cells are encouraged to form aggregates which differentiate forming outer endodermal layers which encapsulate the aggregates.
Aggregates (embryoid bodies) can be encouraged to differentiate further by keeping them in suspension for 5 or more days.
Tested and found negative for ectromelia virus (mousepox).
Derivation
The SCC-PSA1 line was isolated from secondary cultures of the OT/5568 transplantable tumor.
Clinical Data
male
Comments
NOTE - NO LIVE CULTURES CAN BE SENT.
The cells can be maintained in an undifferentiated state by frequent subculture and the use of a fibroblast feeder cell layer (see ATCC CRL-1503).
In the absence of a feeder layer, the cells are encouraged to form aggregates which differentiate forming outer endodermal layers which encapsulate the aggregates.
Aggregates (embryoid bodies) can be encouraged to differentiate further by keeping them in suspension for 5 or more days.
Collect the bodies from a bacteriological dish and plate them in fresh medium in a tissue culture dish without feeder layer or gelatin.
A somewhat variable percentage will attach.
Change the medium every 2 to 3 days.
Migration of the endodermal layer should begin within 24 to 36 hours after plating.
Do not dislodge cells when changing the medium.
The SCC-PSA1 line was isolated from secondary cultures of the OT/5568 transplantable tumor.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Remove medium, add fresh 0.25% trypsin, 0.02% EDTA solution to disperse the cells.
Dilute with fresh medium, centrifuge, and resuspend the cells in fresh medium.
Seed cultures with 8 X 10 4 cells/cm2 in flasks with fibroblast feeder layers (e.g., MITC-STO cells, ATCC 56-X.2).
Subculture every 3 days to maintain in an undifferentiated proliferative state.
Culture Conditions
Temperature: 37.0°C
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Martin GR, Evans MJ. Differentiation of clonal lines of teratocarcinoma cells: formation of embryoid bodies in vitro. Proc. Natl. Acad. Sci. USA 72: 1441-1445, 1975. PubMed: 1055416

Martin GR, et al. The development of cystic embryoid bodies in vitro from clonal teratocarcinoma stem cells. Dev. Biol. 61: 230-244, 1977. PubMed: 590624

Stevens LC. The development of transplantable teratocarcinomas from intratesticular grafts of pre- and postimplantation mouse embryos. Dev. Biol. 21: 364-382, 1970. PubMed: 5436899