Hs27 (ATCC® CRL-1634)

Organism: Homo sapiens, human  /  Cell Type: fibroblast  /  Tissue: skin, foreskin  / 

Organism Homo sapiens, human
Tissue skin, foreskin
Cell Type fibroblast
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Age newborn
Gender male
Ethnicity Black
Storage Conditions liquid nitrogen vapor phase
Clinical Data
Black
male
newborn
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Medium Renewal: 2 to 3 times per week
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 12,14
D16S539: 11,13
D5S818: 12
D7S820: 9,11
THO1: 7,8
TPOX: 6,8
vWA: 18
Isoenzymes
G6PD, A
Name of Depositor RB Owens
References

Suss-Toby E, et al. Toxoplasma invasion: the parasitophorous vacuole is formed from host cell plasma membrane and pinches off via a fission pore. Proc. Natl. Acad. Sci. USA 93: 8413-8418, 1996. PubMed: 8710885

Basic Documentation
References

Suss-Toby E, et al. Toxoplasma invasion: the parasitophorous vacuole is formed from host cell plasma membrane and pinches off via a fission pore. Proc. Natl. Acad. Sci. USA 93: 8413-8418, 1996. PubMed: 8710885