J45.01 (ATCC® CRL-1990)

Organism: Homo sapiens, human  /  Cell Type: T lymphocyte  /  Tissue: peripheral blood  /  Disease: acute T cell leukemia

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Organism Homo sapiens, human
Tissue peripheral blood
Cell Type T lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease acute T cell leukemia
Gender male
Storage Conditions liquid nitrogen vapor phase
Derivation
CD45 deficient cells were isolated using a fluorescence activated cell sorter, and cloned by limiting dilution.
The Jurkat cell line was established from the peripheral blood of a 14 year old boy by Schneider et al., and was originally designated JM.
The line was isolated after gamma irradiation of E6-1 followed by lysis of CD45 positive cells with the GAP 8.3 monoclonal antibody (see ATCC HB-12) and complement.
Clinical Data
The Jurkat cell line was established from the peripheral blood of a 14 year old boy by Schneider et al.
male
Antigen Expression
CD2 +; CD3 +; CD28 +; CD45 -
Receptor Expression
T cell antigen receptor
Genes Expressed
produce interleukin 2 (IL-2) in response to calcium ionophores and phorbol esters
Comments
The J45.01 cell line is a CD45 deficient variant of the E6-1 clone of Jurkat (ATCC TIB-152).
CD45 deficient cells were isolated using a fluorescence activated cell sorter, and cloned by limiting dilution.
The cells express the T cell antigen receptor (TCR, CD3), CD2 and CD28 at levels comparable to the parental line, but only 5 to 8% as much CD45.
Loss of CD45 diminishes TCR - CD2 signal transduction, thus the cells are inducible for interleukin 2 (IL-2) production with calcium ionophores and phorbol esters, but not with TCr or CD2 stimulation.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 105 cells/mLand maintain between 1 X 105 and 1 X 106 cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze Medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage Temperature: Liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
Name of Depositor GA Koretsky
References

Koretzky GA, et al. Tyrosine phosphatase CD45 is required for T-cell antigen receptor and CD2-mediated activation of a protein tyrosine kinase and interleukin 2 production. Proc. Natl. Acad. Sci. USA 88: 2037-2041, 1991. PubMed: 1672451

Schneider U, et al. Characterization of EBV-genome negative "null" and "T" cell lines derived from children with acute lymphoblastic leukemia and leukemic transformed non-Hodgkin lymphoma. Int. J. Cancer 19: 621-626, 1977. PubMed: 68013

Ronald Wange, personal communication

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Koretzky GA, et al. Tyrosine phosphatase CD45 is required for T-cell antigen receptor and CD2-mediated activation of a protein tyrosine kinase and interleukin 2 production. Proc. Natl. Acad. Sci. USA 88: 2037-2041, 1991. PubMed: 1672451

Schneider U, et al. Characterization of EBV-genome negative "null" and "T" cell lines derived from children with acute lymphoblastic leukemia and leukemic transformed non-Hodgkin lymphoma. Int. J. Cancer 19: 621-626, 1977. PubMed: 68013

Ronald Wange, personal communication