J.CaM1.6 (derivative mutant of Jurkat) (ATCC® CRL-2063)

Organism: Homo sapiens, human  /  Cell Type: T lymphocyte  /  Tissue: Peripheral blood  /  Disease: acute T cell leukemia

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Organism Homo sapiens, human
Tissue Peripheral blood
Cell Type T lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease acute T cell leukemia
Gender male
Applications This cell line is a suitable transfection host.
Storage Conditions liquid nitrogen vapor phase
Derivation
The J.CaM1.6 cell line is a derivative mutant of Jurkat. The Jurkat cell line was established from the peripheral blood of a 14 year old boy by Schneider et al., and was originally designated JM. The J.CaM1.6 line was derived from the E6-1 clone of Jurkat (ATCC TIB-152) by treatment with ethylmethanesulfonate (EMS). Cells were then selected for T cell antigen receptor (TCR) expression and inability to increase intracellular calcium in response to TCR stimulation with OKT3 antibody.
Clinical Data
male
14 years
Comments CD3/T1 complexes are present; however, TCR signal transduction is defective. They are deficient in lck kinase activity and are missing exon 7 in their lck mRNA. The T cell antigen receptor appears to be structurally normal.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 105 viable cells/mL. Maintain cell density between 2 X 105 and 2 X 106 viable cells/mL.
Medium Renewal: Twice per week
Cryopreservation
Freeze Medium: Complete growth medium, 95%; DMSO, 5%
Storage Temperature: Liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 11
D13S317: 8,12
D16S539: 10,11
D5S818: 9
D7S820: 8,10
THO1: 6,9.3
TPOX: 8,10
vWA: 18,19
Name of Depositor A Weiss
References

Straus DB, Weiss A. Genetic evidence for the involvement of the lck tyrosine kinase in signal transduction through the T cell antigen receptor. Cell 70: 585-593, 1992. PubMed: 1505025

Goldsmith MA, Weiss A. Isolation and characterization of a T-lymphocyte somatic mutant with altered signal transduction by the antigen receptor. Proc. Natl. Acad. Sci. USA 84: 6879-6883, 1987. PubMed: 3309950

Lund T, et al. Herpesvirus saimiri Tip-484 membrane protein markedly increases p56lck activity in T cells. J. Virol. 71: 378-382, 1997. PubMed: 8985360

Cenciarelli C, et al. T cell antigen receptor ubiquitination is a consequence of receptor-mediated tyrosine kinase activation. J. Biol. Chem. 271: 8709-8713, 1996. PubMed: 8621503

Gibson S, et al. Functional LCK is required for optimal CD28-mediated activation of the TEC family tyrosine kinase EMT/ITK. J. Biol. Chem. 271: 7079-7083, 1996. PubMed: 8636141

Hutchcroft JE, et al. Differential phosphorylation of the T lymphocyte costimulatory receptor CD28. J. Biol. Chem. 271: 13362-13370, 1996. PubMed: 8662792

Schneider U, et al. Characterization of EBV-genome negative "null" and "T" cell lines derived from children with acute lymphoblastic leukemia and leukemic transformed non-Hodgkin lymphoma. Int. J. Cancer 19: 621-626, 1977. PubMed: 68013

Ronald Wange, personal communication

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Straus DB, Weiss A. Genetic evidence for the involvement of the lck tyrosine kinase in signal transduction through the T cell antigen receptor. Cell 70: 585-593, 1992. PubMed: 1505025

Goldsmith MA, Weiss A. Isolation and characterization of a T-lymphocyte somatic mutant with altered signal transduction by the antigen receptor. Proc. Natl. Acad. Sci. USA 84: 6879-6883, 1987. PubMed: 3309950

Lund T, et al. Herpesvirus saimiri Tip-484 membrane protein markedly increases p56lck activity in T cells. J. Virol. 71: 378-382, 1997. PubMed: 8985360

Cenciarelli C, et al. T cell antigen receptor ubiquitination is a consequence of receptor-mediated tyrosine kinase activation. J. Biol. Chem. 271: 8709-8713, 1996. PubMed: 8621503

Gibson S, et al. Functional LCK is required for optimal CD28-mediated activation of the TEC family tyrosine kinase EMT/ITK. J. Biol. Chem. 271: 7079-7083, 1996. PubMed: 8636141

Hutchcroft JE, et al. Differential phosphorylation of the T lymphocyte costimulatory receptor CD28. J. Biol. Chem. 271: 13362-13370, 1996. PubMed: 8662792

Schneider U, et al. Characterization of EBV-genome negative "null" and "T" cell lines derived from children with acute lymphoblastic leukemia and leukemic transformed non-Hodgkin lymphoma. Int. J. Cancer 19: 621-626, 1977. PubMed: 68013

Ronald Wange, personal communication