HCC1187 (ATCC® CRL-2322)

Organism: Homo sapiens, human  /  Cell Type: epithelial  /  Tissue: mammary gland; breast  /  Disease: TNM stage IIA, grade 3, primary ductal carcinoma

Permits and Restrictions

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Organism Homo sapiens, human
Tissue mammary gland; breast
Cell Type epithelial
Product Format frozen
Morphology epithelial
Culture Properties mixed, adherent and suspension
Biosafety Level 1
Disease TNM stage IIA, grade 3, primary ductal carcinoma
Age 41 years adult
Gender female
Ethnicity Caucasian, White
Storage Conditions liquid nitrogen vapor phase
Karyotype multiploid; cell population has several ploidy indices
Derivation
This cell line was initiated on 9/13/94 from a patient who had received prior chemotherapy. The cell line took 4.5 months to establish.
Clinical Data
41 years
Caucasian
female

Receptor Expression
progesterone receptor, negative
Oncogene her2/neu -, p53 +
Cellular Products
Epithelial glycoprotein 2 (EGP2)
cytokeratin 19
Comments
The tumor was classified as TNM stage IIA, grade 3, invasive ductal carcinoma. Information regarding lymph node metastasis is not available.
The cells are poorly differentiated.

An EBV transformed lymphoblastoid cell line (HCC1187 BL) from the same patient is available as ATCC CRL-2323.

HCC1187 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 (EGP2) and for cytokeratin 19. The cells are negative for expression of Her2-neu; they overexpress p53. The cells are negative for expression of progesterone receptor (PR).

Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.


Phenol Red is a structural mimic for estrogen. Therefore the estrogenic activity of phenol red should be considered in any studies that utilize estrogen-responsive cells in culture (RefBerthois Y, et al. Phenol red in tissue culture media is a weak estrogen: implications concerning the study of estrogen-responsive cells in culture. Proc. Natl. Acad. Sci. USA 83: 2496–2500, 1986. PubMed: 3458212). The Phenol Red-free version of RPMI 1640, is ATCC catalog No. 30-2602.

Subculturing

Cultures grow as floating aggregates of round cell clusters and as attached epithelial-like cells. Cultures can be maintained by the addition of fresh medium to the flask, or centrifuging and removing the spent medium, resuspending the cell pellet in fresh medium and returning the resuspended cells to the flask.


To subculture the attached cells, remove and reserve the suspended cells (recover the suspended cells by centrifugation if desired). Rinse the monolayer with fresh 0.25% trypsin-0.53 mM EDTA, neutralize with an equal volume complete medium and centrifuge. Remove supernatant and resuspend cell pellet with complete medium and pool into reserved suspended cells. Add appropriate aliquots of the pooled cell suspension to new culture vessels.


Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile
Amelogenin: X
CSF1PO: 13
D13S317: 11
D16S539: 10
D5S818: 12
D7S820: 8,11
THO1: 6
TPOX: 8
vWA: 19
Name of Depositor AF Gazdar, AK Virmani
References

Gazdar AF, et al. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PubMed: 9833771

Basic Documentation
Other Documentation
Restrictions

The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. Adi F. Gazdar and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 648-1888, Email TechnologyDevelopment@UTSouthwestern.edu, or Fax: (214) 951-0935.

References

Gazdar AF, et al. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PubMed: 9833771