HCC1419 (ATCC® CRL-2326)

Organism: Homo sapiens, human  /  Cell Type: Epithelial  /  Tissue: mammary gland; breast  /  Disease: TNM stage IIIA, grade 3,primary ductal carcinoma

Permits and Restrictions

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Organism Homo sapiens, human
Tissue mammary gland; breast
Cell Type Epithelial
Product Format frozen
Morphology epithelial
Culture Properties adherent, The line grows as large epithelial attached cells in island-like formation.
Biosafety Level 1
Disease TNM stage IIIA, grade 3,primary ductal carcinoma
Age 42 years
Gender female
Ethnicity Hispanic
Storage Conditions liquid nitrogen vapor temperature
Karyotype aneuploid
Derivation
This cell line was initiated on 12/28/94 from a patient who received prior chemotherapy with no previous family history of breast cancer. The line took 9 months to establish.
Clinical Data
female
Hispanic
42 years
Receptor Expression
estrogen receptor, negative
progesterone receptor, negative
Oncogene her2/neu +, p53 -
Genes Expressed
Epithelial glycoprotein 2 [EGP2]; cytokeratin 19
Cellular Products
Epithelial glycoprotein 2 [EGP2]; cytokeratin 19
Comments

The tumor was classified as TNM stage IIIA, grade 3, invasive ductal carcinoma with 5 out of 5 lymph node metastasis. 

The cells are poorly differentiated. 

The cells overexpress Her2-neu but are negative for expression of p53. HCC1419 is positive for the epithelial cell specific marker epithelial glycoprotein 2 (EGP2) and for cytokeratin 19. 

The cells are negative for expression of estrogen receptor (ER) and for expression of progesterone receptor (PR).


Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 10 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C

Subculture Ratio: 1:2 to 1:3
Medium Renewal: 2 to 3 times a week.
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 5th edition, published by Wiley-Liss, N.Y., 2005.

Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
Growth Conditions: The line grows as large epithelial attached cells in island-like formation.
STR Profile
Amelogenin: X
CSF1PO: 10,12
D13S317: 9,12
D16S539: 11,12
D5S818: 10,12
D7S820: 11
THO1: 6,9.3
TPOX: 8
vWA: 16,17
Name of Depositor AF Gazdar, AK Virmani
Year of Origin December 28, 1994
References

Gazdar AF, et al. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PubMed: 9833771

Basic Documentation
Restrictions

The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. Adi F. Gazdar and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 648-1888, Email TechnologyDevelopment@UTSouthwestern.edu, or Fax: (214) 951-0935.

References

Gazdar AF, et al. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PubMed: 9833771