293/CHE-Fc (ATCC® CRL-2368)

Organism: Homo sapiens, human  /  Disease: Influenza

Organism Homo sapiens, human
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 2 CELLS CONTAIN ADENOVIRUS
Disease Influenza
Age fetus
Applications
293/CHE-Fc was established by cotransfecting human embryonic 293 cell (ATCC CRL-1573) with the plasmid pCHE-Fc and a plasmid encoding Genetic (G418) resistance.
The cell line is stably transfected to secrete the chimeric protein (CHE-Fc), a recombinant soluble chirmeric protein useful as a probe to detect 9-O-Acetylated sialic acids.
CHE-Fc is a bifunctional molecule that can be used either to cleave acetyl groups at the ninth position of sialic acids or as a binding probe for 9-O-sialic acids.
Derivation
293/CHE-Fc was established by cotransfecting human embryonic 293 cell (ATCC CRL-1573) with the plasmid pCHE-Fc and a plasmid encoding Genetic (G418) resistance.
Genes Expressed
soluble CHE-Fc
Cellular Products
soluble CHE-Fc
Comments
293/CHE-Fc was established by cotransfecting human embryonic 293 cell (ATCC CRL-1573) with the plasmid pCHE-Fc and a plasmid encoding Genetic (G418) resistance.
The cell line is stably transfected to secrete the chimeric protein (CHE-Fc), a recombinant soluble chirmeric protein useful as a probe to detect 9-O-Acetylated sialic acids.
CHE-Fc is the fusion protein of hemagglutinin-esterase from Influenza C with human IgG1 Fc.
CHE-Fc is a bifunctional molecule that can be used either to cleave acetyl groups at the ninth position of sialic acids or as a binding probe for 9-O-sialic acids.
293/CHE-Fc cells are G418 resistant.
Complete Growth Medium Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 1 mg/ml G-418, 90%; heat inactivated fetal bovine serum, 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:10 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Population Doubling Time 24 hrs
Name of Depositor AP Varki
References

Klein A, et al. 9-O-Acetylated sialic acids have widespread but selective expression: Analysis using a chimeric dual-function probe derived from influenza C hemaggIutinin-estrase. Proc. Natl. Acad. Sci. USA 91: 7782-7786, 1994. PubMed: 8052660

Basic Documentation
References

Klein A, et al. 9-O-Acetylated sialic acids have widespread but selective expression: Analysis using a chimeric dual-function probe derived from influenza C hemaggIutinin-estrase. Proc. Natl. Acad. Sci. USA 91: 7782-7786, 1994. PubMed: 8052660