TF-1a

The cells retain the ability to respond to a variety of cytokines, with a different response pattern from the parental cell line.

TF-1a, but not TF-1 cells, form colonies in soft agar culture in the absence of any added growth factors, and generate invasive tumors in nude mice.

There is a slight constitutive activation of the MAP kinase and MEK proteins in TF-1a but not in TF-1 cells. Phenotypically, TF-1 cells are CD34 positive and CD38 positive, whereas TF-1a cells are CD34 positive and CD38 negative.

TF1-a cells, but not TF-1 cells, are resistant to tumor necrosis factor alpha (TNF-alpha) induced apoptosis.

It can be used to study signal pathways involved in the spontaneous and factor-induced growth of the cells.

A culture submitted to the ATCC in April of 1999 was found to be contaminated with mycoplasma. Progeny were cured by a 21-day treatment with BM Cycline.

1. Check all containers for leakage or breakage.
2. Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below ­-130°C, preferably in liquid nitrogen vapor, until ready for use.

1. Thaw the vial by gentle agitation in a 37°C water bath. To reduce the possibility of contamination, keep the O-ring and cap out of the water.  Thawing should be rapid (approximately 2 minutes).
2. Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by dipping in or spraying with 70% ethanol. All of the operations from this point on should be carried out under strict aseptic conditions.
3. Transfer the vial contents to a centrifuge tube containing  9.0 mL complete culture medium and spin at approximately 125 x g for 5 to 7 minutes. It is important to avoid excessive alkalinity of the medium during recovery of the cells.  It is suggested that, prior to the addition of the vial contents, the culture vessel containing the complete growth medium be placed into the incubator for at least 15 minutes to allow the medium to reach its normal pH (7.0 to 7.6).
4. Incubate the culture at 37°C in a suitable incubator.  A 5% CO₂ in air atmosphere is recommended if using the medium described on this product sheet.

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