Neo Jurkat (ATCC® CRL-2898)

Organism: Homo sapiens, human  /  Cell Type: T-Cell/ T Lymphocyte  /  Tissue: peripheral blood  /  Disease: Acute T-Cell Leukemia

Organism Homo sapiens, human
Tissue peripheral blood
Cell Type T-Cell/ T Lymphocyte
Product Format frozen
Morphology lymphoid-like
Culture Properties suspension
Biosafety Level 2
Disease Acute T-Cell Leukemia
Age 14 years
Gender Male
Applications

When paired with ATCC CRL-2899, ATCC CRL-2900, ATCC CRL-2901 or ATCC CRL-2902, this cell line can be used as a control cell line to study BCL2 function and its signaling pathway.

Storage Conditions liquid nitrogen vapor phase
Images CRL-2898 Micrograph
Derivation The Neo Jurkat cell line was derived by transfecting human Jurkat T cells with the empty pSFFV mammalian expression vector carrying a neomycin-resistant gene.  Stable neomycin (Neo)-resistant single-cell-originated clones were isolated and expanded by selection in medium containing 1 mg/mL G418 for 14 days.
Genes Expressed

Neomycin resistance gene expressed

Tumorigenic Yes
Comments

The Neo Jurkat cell line was derived by transfecting human Jurkat T cells with the empty pSFFV mammalian expression vector carrying a neomycin-resistant gene.  Stable neomycin (Neo)-resistant single-cell-originated clones were isolated and expanded by selection in medium containing 1 mg/mL G418 for 14 days.

Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: 10% FBS and 200 mcg/mL G418.
Subculturing

Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium. An inoculum of 2 x 105 to 3 x 105 cells/mL is recommended. Subculture when cell concentration is 2 x 106cells/mL.

Subcultivation ratio: A subcultivation ratio of 1:2 to 1:10 is recommended.
Medium renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Culture Conditions Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile
D5S818: 9
D13S317: 8, 12
D7S820: 8, 12
D16S539: 11
vWA: 18
TH01: 6, 9.3
Amelogenin: X Y
TPOX: 8, 10
CSF1PO: 11, 12

Name of Depositor Stanley Korsmeyer
Year of Origin 2002
References

Yamamoto K, et al. BCL-2 is Phosphorylated and Inactivated by an ASK1/Jun N-Terminal Protein Kinase Pathway normally Activated at G2/M. Mol Cell Biol. Dec;19(12):8469-78. 1999. PubMed: 10567572

Basic Documentation
Other Documentation
References

Yamamoto K, et al. BCL-2 is Phosphorylated and Inactivated by an ASK1/Jun N-Terminal Protein Kinase Pathway normally Activated at G2/M. Mol Cell Biol. Dec;19(12):8469-78. 1999. PubMed: 10567572