WIL2-S (ATCC® CRL-8885)

Organism: Homo sapiens, human  /  Cell Type: B lymphoblast  /  Tissue: spleen  /  Disease: hereditary spherocytosis

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Organism Homo sapiens, human
Tissue
spleen
Cell Type B lymphoblast
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 2  [Cells contain Epstein-Barr virus]
Disease hereditary spherocytosis
Age 5 years
Gender male
Ethnicity Caucasian
Applications
This cell line is a suitable transfection host.
Storage Conditions liquid niotrogen vapor phase
Clinical Data
5 years
Caucasian
male
Comments
This is a HAT sensitive variant of the WIL2 human B cell line (see also ATCC CRL-8155).
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 105 cells/mL and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/mL.
Medium Renewal: Every 2 to 3 days
STR Profile
Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 11
D16S539: 11,12
D5S818: 12,13
D7S820: 9,11
F13A01: 6,7
F13B: 10
FESFPS: 11,12
LPL: 9,10
THO1: 8,9.3
TPOX: 8,11
vWA: 17,20
Name of Depositor Lovelace Medical Foundation
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation