8E5 [derivative of CEM] (ATCC® CRL-8993)

Organism: Homo sapiens, human  /  Cell Type: lymphoblast human immunodeficiency virus (HIV) positive  /  Tissue: peripheral blood  /  Disease: acute lymphoblastic leukemia

Organism Homo sapiens, human
Tissue peripheral blood
Cell Type lymphoblast human immunodeficiency virus (HIV) positive
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 2  [Cells contain Retrovirus]
Disease acute lymphoblastic leukemia
Age 4 years
Gender female
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Clinical Data female
Caucasian
4 years
Antigen Expression
CD4 +; CD5 +
Receptor Expression
transferrin
Genes Expressed
most human immunodeficiency virus (HIV, HTLV III, LAV) structural proteins
Cellular Products
most human immunodeficiency virus (HIV, HTLV III, LAV) structural proteins
Comments

The cells contain a single defective proviral genome of HTLV III (HIV, LAV).

All of the major structural protein are constitutively expressed with the exception of the p64 and p34 proteins.

No infectious virus is produced; however, co-cultivation with Leu-3 + cells leads to syncytia formation.

The cells have been cured of a mycoplasma contamination.

Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 10viable cells/mL. Maintain cultures at a cell concentration between 1 x 105 and 1 x 10cells/mL.
Medium Renewal: Add fresh medium (20% to 30% by volume) every 2 to 3 days.
Cryopreservation
Complete growth medium 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions
Temperature: 37°C
Name of Depositor The United States of America
References

Powell DM, et al. Cell line producing AIDS viral antigens without producing infectious virus particles. US Patent 4,752,565 dated Jun 21 1988

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online at http://www.cdc.gov/OD/ohs/biosfty/bmbl5/bmbl5toc.htm

Basic Documentation
References

Powell DM, et al. Cell line producing AIDS viral antigens without producing infectious virus particles. US Patent 4,752,565 dated Jun 21 1988

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online at http://www.cdc.gov/OD/ohs/biosfty/bmbl5/bmbl5toc.htm