High molecular weight genomic DNA isolated from RPMI 8226; Plasmacytoma; Human cell line. (ATCC® CRM-CCL-155D)

Organism: Homo sapiens, human  / 

Product Format 5 μg
Storage Conditions -80°C or colder
Applications

For use in testing and calibration in ISO 17025 accredited laboratories, to challenge assay performance, validate or compare test methods, and to establish sensitivity, linearity and specificity during assay validation or implementation. ISO Guide 34:2009.

Comments

Store at   - 80°C or colder in a manual defrost freezer.  Avoid freeze-thawing.  Alternatively, product can be stored at -20°C or colder, but stability at this temperature has not been tested by the ATCC and is not guaranteed.

Certified Reference Material produced under an ISO Guide 34:2009 accredited process.

This item has been tested for KRAS mutation: Heterozygous DNA Change: c.35G>C (correlates to protein sequence p.G12A)

Intended Use

Certified Reference Material produced under an ISO Guide 34:2009 accredited process. 


    ACLASS Accredited Reference Material Producer AR-1384

Biosafety Level 1
Quality Control Specifications

Certified Reference Material produced under an ISO Guide 34:2009 accredited process.

Test

Specification

Total DNA amount

Average of three (3) vials tested:

≥ 5 µg/vial

Undiluted DNA concentration

Average of three (3) vials tested:

100 ng/µL (± 15%)

Purity (A260/A280)

Average of three (3) vials tested:

1.7 to 2.1

Electrophoresis - RNA content

No visible RNA

Electrophoresis - DNA molecular weight 

Uncut DNA band is ≥48 kb or higher than the uppermost band of the high molecular weight DNA ladder

Electrophoresis - DNA digestion

DNA is digested by BamHI to a downward smear and is lower than the uncut DNA

Certificates of Analysis are available electronically at www.atcc.org, or by hardcopy upon request.