F18 AF1 (ATCC® HB-8208)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Derivation
Animals were immunized with purified CSF-I.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
CSF-I was purified from medium conditioned by MIA PaCa-2 (ATCC CRL-1420).
Genes Expressed
immunoglobulin; monoclonal antibody; against human colony stimulating factor subclass I (CSF-I)
Tumorigenic Yes
Effects
Yes, forms ascites in pristane primed BALB/c mice
Comments
Animals were immunized with purified CSF-I.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
CSF-I was purified from medium conditioned by MIA PaCa-2 (ATCC CRL-1420).
Complete Growth Medium HB101 medium (Hana Biologics), 99%; heat-inactivated fetal bovine serum, 1%
Complete Growth Medium
Culture Medium: Iscove's modified Dulbecco's medium, 90%; fetal bovine serum,
10%.
Subculturing
Medium Renewal: Twice per week
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 X 10 exp4 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgG1
Name of Depositor Amgen, Inc.
References

Nicolson M. Hybridoma tumor cell lines and their monoclonal antibodies to human colony stimulating factor subclass number 1. US Patent 4,504,586 dated Mar 12 1985

. . Hybridoma 2: 137, 1983.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Nicolson M. Hybridoma tumor cell lines and their monoclonal antibodies to human colony stimulating factor subclass number 1. US Patent 4,504,586 dated Mar 12 1985

. . Hybridoma 2: 137, 1983.