MDA-MB-436 (ATCC® HTB-130)

Organism: Homo sapiens, human  /  Tissue: mammary gland/breast; derived from metastatic site: pleural effusion  /  Disease: adenocarcinoma

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Organism Homo sapiens, human
Tissue
mammary gland/breast; derived from metastatic site: pleural effusion
Product Format frozen
Morphology pleomorphic with multinucleated component cells
Culture Properties adherent
Biosafety Level 1
Disease adenocarcinoma
Age 43 years
Gender female
Ethnicity Caucasian
Karyotype modal number = 45
Clinical Data
43 years
Caucasian
female
Genes Expressed
tubulin; actin
Cellular Products Cellular products: tubulin, actin
Tumorigenic No
Effects
No, in immunosuppressed mice
Yes, in semisolid medium
Comments
The line is pleomorphic and most cells react intensely with anti tubulin antibody as demonstrated by indirect immunofluorescence staining.
Complete Growth Medium Leibovitz's L-15 medium with 10 mcg/ml insulin, 16 mcg/ml glutathione, 90%; fetal bovine serum, 10%.

(Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation)


Subculturing
Subcultures are prepared by scraping. Remove spent medium, add 3 to 5 ml of fresh medium, dislodge cells from the floor of the flask, aspirate and dispense into new flasks. Subculture every 6 to 8 days.
Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Culture medium, 95%; DMSO, 5%
Culture Conditions
Atmosphere: air, 100%

Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 12
D13S317: 10
D16S539: 9,11
D5S818: 13
D7S820: 10
THO1: 9.3
TPOX: 8
vWA: 14,20
Isoenzymes
AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 2
Me-2, 1
PGM1, 1
PGM3, 1
Name of Depositor R Cailleau
References

Brinkley BR, et al. Variations in cell form and cytoskeleton in human breast carcinoma cells in vitro. Cancer Res. 40: 3118-3129, 1980. PubMed: 7000337

Siciliano MJ, et al. Mutually exclusive genetic signatures of human breast tumor cell lines with a common chromosomal marker. Cancer Res. 39: 919-922, 1979. PubMed: 427779

Cailleau R, et al. Long-term human breast carcinoma cell lines of metastatic origin: preliminary characterization. In Vitro 14: 911-915, 1978. PubMed: 730202

Lan MS, et al. Polypeptide core of a human pancreatic tumor mucin antigen. Cancer Res. 50: 2997-3001, 1990. PubMed: 2334903

Basic Documentation
Other Documentation
Restrictions

The ATCC Material is available with the following restrictions: The ATCC Material may not be used for commercial purposes. Purchaser will not make commercial use of the ATCC Material without obtaining a license from the University of Texas MD Anderson Cancer Center (“UTMDACC”). With respect to research use, prior to purchase, for-profit or commercial entities must first obtain a research use license from UTMDACC. For instructions on how to proceed, please contact Dustin J. Romine, M.A. at the UTMDACC Office of Technology Commercialization via email at dromine@mdanderson.org.

References

Brinkley BR, et al. Variations in cell form and cytoskeleton in human breast carcinoma cells in vitro. Cancer Res. 40: 3118-3129, 1980. PubMed: 7000337

Siciliano MJ, et al. Mutually exclusive genetic signatures of human breast tumor cell lines with a common chromosomal marker. Cancer Res. 39: 919-922, 1979. PubMed: 427779

Cailleau R, et al. Long-term human breast carcinoma cell lines of metastatic origin: preliminary characterization. In Vitro 14: 911-915, 1978. PubMed: 730202

Lan MS, et al. Polypeptide core of a human pancreatic tumor mucin antigen. Cancer Res. 50: 2997-3001, 1990. PubMed: 2334903